Can I please get help on proposing the next experiment (future direction) for the information provided below? Reference: MicroRNA-9 regulates neural apoptosis in methylmalonic acidemia via targeting BCL2L11 AbstractMethylmalonic acidemia (MMA) is an autosomal-recessiveinborn metabolic disorder that results from a deficiency inmethylmalonyl-coenzyme A mutase or its cofactor,adenosylcobalamin. Currently, neurological manifestations inMMA are thought to be associated with neural apoptosis.BCL2L11, which is a proapoptotic Bcl-2 family member, isresident in the outer mitochondrial membrane, where thisprotein acts as a central regulator of the intrinsic apoptoticcascade and mediates excitotoxic apoptosis. MicroRNAs(miRNAs) are a class of non-coding RNAS that function asendogenous triggers of the RNA interference pathway.Currently, little is known regarding the role of miRNA inMMA. In our previous study, we preliminarily found that theexpression of miR-9 was significantly down-regulated inMMA patient plasma and sensitively changed after VitB12treatment, which may act as a potential "competitor" ofchromatography-mass spectrometry for the diagnosis ofMMA. In the present study, we first confirmed that miR-9inhibited BCL2L11 expression by directly targeting its 3'-gasuntranslated region, and the up-regulation of miR-9 reducedneural apoptosis induced by methylmalonate via targetingBCL2L11. Taken together, our results suggested that miR-9might act as a monitor of changes in MMA and mightprovide new insights into a therapeutic entry point fortreating MMA.

Name: Can I please get help on proposing the next experiment (future directi
Uploaded: 2024-03-20T06:42:55.000Z
Channel: Bartleby
Description: Can I please get help on proposing the next experiment (future direction) for the information provided below? Reference: MicroRNA-9 regulates neural apoptosis in methylmalonic acidemia via targeting BCL2L11 AbstractMethylmalonic acidemia (MMA) is an