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A single mutation in the riboswitch’s sequence changes the ligand binding specificity from guanine to adenine. Predict what single mutation could be made in the guanine-responsive riboswitch sequence to convert it to an adenine-responsive riboswitch. Justify your answer b. Could a DNA molecule with the same nucleotide sequence as the guanine-responsive riboswitch bind guanine in a similar manner as the RNA-based molecule? Explain

A single mutation in the riboswitch’s sequence changes the ligand binding specificity from guanine to adenine. Predict what single mutation could be made in the guanine-responsive riboswitch sequence to convert it to an adenine-responsive riboswitch. Justify your answer b. Could a DNA molecule with the same nucleotide sequence as the guanine-responsive riboswitch bind guanine in a similar manner as the RNA-based molecule? Explain

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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a. A single mutation in the riboswitch’s sequence changes the ligand binding specificity from guanine to
adenine. Predict what single mutation could be made in the guanine-responsive riboswitch sequence
to convert it to an adenine-responsive riboswitch. Justify your answer

b. Could a DNA molecule with the same nucleotide sequence as the guanine-responsive riboswitch bind
guanine in a similar manner as the RNA-based molecule? Explain

6. Riboswitches are RNA sequences found in the 5' untranslated region of messenger RNA (mRNA) that control gene expression in response to small molecule binding. These regulatory elements adopt two conformations: an "on" state when a specific small molecule ligand is bound and an "off" state in the absence of ligand. When the mRNA is "off", the gene remains untranslated. The structure of a guanine-responsive riboswitch was solved in 2004 (Nature, 432, 411-415) and is shown below at low (left) and high (right) resolution with a guanine derivative bound: (see Blackboard for color image) Guanine riboswitch with a guanine derivative (red) bound; zoomed out. U22 U47 HX U51 C74 Guanine riboswitch with a guanine derivative (HX) bound; atomic detail. Note: blue = nitrogen, red = oxygen. C and U follow standard nucleotide naming conventions.
Transcribed Image Text:6. Riboswitches are RNA sequences found in the 5' untranslated region of messenger RNA (mRNA) that control gene expression in response to small molecule binding. These regulatory elements adopt two conformations: an "on" state when a specific small molecule ligand is bound and an "off" state in the absence of ligand. When the mRNA is "off", the gene remains untranslated. The structure of a guanine-responsive riboswitch was solved in 2004 (Nature, 432, 411-415) and is shown below at low (left) and high (right) resolution with a guanine derivative bound: (see Blackboard for color image) Guanine riboswitch with a guanine derivative (red) bound; zoomed out. U22 U47 HX U51 C74 Guanine riboswitch with a guanine derivative (HX) bound; atomic detail. Note: blue = nitrogen, red = oxygen. C and U follow standard nucleotide naming conventions.
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