Answered: Wilms tumor 1, or nephroblastoma, is…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Related questions

Question

Wilms tumor 1, or nephroblastoma, is caused by mutations in the WT1 gene, which encodes a transcription factor. You have identified a novel variant in WT1: Arg422Pro.

You have control cells and cells that have been engineered to carry the homozygous WT1 p.Arg422Pro mutation. You want to assess effects of this mutation on a variety of endpoints. For each endpoint listed below, choose the one technique is best suited to answer the question.

Choose from: array CGH, qRT-PCR, qPCR, RNA-seq, FISH, in situ hybridization, western blot, immunostaining, WT1 ChIP-seq, WT1 ChIP-PCR, ATAC-seq, 3C

Endpoint	Technique?
WT1 protein amount (quantitative)	Western blot
WT1 protein binding to all enhancers, genome-wide	Chip-seq
WT1 mRNA amount (quantitative)
WT1 protein subcellular localization
Quantitative assessment of all mRNAs in these cells (genome-wide)	RNAseq
Chromatin interactions between a specific WT1 chromatin binding site (identified above) and a nearby promoter	3C
Open chromatin, genome-wide	ATAcseq