iRNAs are used to “knockdown” gene expression in research. Imagine you are a scientist who hopes to study several genes related to cancer. Select all the scenarios you will be able to successfully use RNA interference with the use of siRNAs (select all that apply): Group of answer choices Regulate transcript levels by targeting the siRNA to core promoter regions (e.g. TATA box) of tumor suppressor genes Regulate transcript levels by targeting the siRNA to the regulatory promoter regions (e.g. enhancers) of tumor suppressor genes Regulate transcript levels by targeting the siRNA to any region of the processed mRNA Regulate transcript levels by targeting the siRNA to the 5’ UTR of the processed mRNA Regulate transcript levels by targeting the siRNA to the 3’ UTR of the processed mRNA Regulate transcript levels by targeting the siRNA to sites where the histone acetyl transferase will acetylate the histones Regulate transcript levels by targeting the siRNA to where the start codon is

Q: Tỏ idéntify transcriptional regulatory elements, reporters containing 100 base pairs of Promoter…

A: Reporter genes help us to understand and determine the expression of gene of interest by fusing them…

Q: This image shows the alignment of the promoter from the same gene (gene H) across multiple mammalian…

A: Introduction  The central promoter region of genes in archaea and eukaryotes contain a DNA sequence…

Q: Label the diagram to explain how the ncRNA HOTAIR plays a role in gene expression. Nearby histones…

A: A long non-coding RNA (nc RNA) molecule is HOTAIR (HOX Transcript Antisense Intergenic RNA). A type…

Q: To identify transcriptional regulatory elements, reporters containing 100 base pairs of Promoter…

A: Transcriptional regulatory elements are the genetic segments that act as the binding sites for…

Q: Match each of the following descriptions to the type of regulatory RNA affecting a "target" gene…

A: Of course! Let's break down each match:This regulatory RNA may increase transcription of the target…

Q: . One mechanism by which antisense RNAs act as negative regulators of gene expression is by base…

A: The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary…

Q: Q.No.6 Northern blotting is used to determine the amount and size of a particular RNA transcribed in…

A: Among a mixture of diverse RNA, through the northern blot transfer technique, specific RNA sequences…

Q: Use the illustration as a reference to select the correct statements transcription factors of…

A: Transcription is the process that is responsible for the production of RNA from a DNA template. It…

Q: Regulation of transcription is mediated by proteins that first must bind to specific…

A: Hi dear,here is your answer. Gene expression is defined as the process by which the information that…

Q: You have isolated a protein that binds to DNA in the region upstream of the promoter sequence of a…

A: The promoter is the site of RNA polymerase binding that is very important step in the initiation…

Q: As discussed in Lipids 3, SREBPs are a type of transcription factor involved in lipid homeostasis.…

A: The full form of  SREBPs is Sterol regulatory element binding proteinsSREBPsare composed of two…

Q: A second hit might occur through epigenetic modification. There is a CpG island in the RB1 promoter.…

A: CpG islands are regions of DNA that are rich in CpG dinucleotides, and they are often located near…

Q: You are studying a new gene that is expressed within the adipose tissue of humans during the…

A: "Genes" can store genetic information in the form of DNA, which may be converted into functional…

Q: radioactively labeled cDNA is used to probe RNA isolated from kidney by Northern blotting. Two bands…

A: Northern blotting is a laboratory technique used to detect and analyze specific RNA molecules. It…

Q: Comparison of different tissues shows that the ITY gene produces a 900 nt mature mRNA and a 190…

A: Alternative splicing is the mechanism that produces multiple mature mRNAs from a single primary…

Q: Examples of cis-acting elements include promoters and enhancers.   true   false

A: Deoxyribonucleic acid (DNA) stores the cell’s genetic information and is present in the nucleus of…

Q: You expect that a lung tumor cell line has arisen due to aberrant expression of the growth factor…

A: Cancer is due to the uncontrolled proliferation of the cell. This uncontrolled proliferation is…

Q: The eukaryotic chromosomes are linear DNA molecules with free 3'-OH and 5'-PO3- ends. DNA free ends…

A: According to our guidelines we are allowed to do one question or up to three sub apart of a…

Q: The figure below shows 6 genes that are differentially expressed based on their specific enhancers.…

A: Enhancers are the regulatory sequences in the DNA located farther away from the transcription start…

Q: a. Use circles for activators and squares for repressors. Draw the shapes bound to each promoter…

A: Transcription - The process by which the information on one strand of DNA is copied to mRNA is…

Q: You are interested in finding out in which organ GTF2H5 is highly expressed experimentally. Describe…

A: Recombinant DNA also written as rDNA is formed by joining or combining the DNA from different…

Q: How does reverse methylation affect gene expression? Select one: o a. The gene is turned off, but…

A: Hello. Since your question has multiple parts, we will solve first question for you. If you want…

Q: Numerous diseases are treated by injection of polypeptide growth factors, such as human growth…

A: Scientists have modified the human cDNA of the growth factor to possibly improve its expression in…

Q: Describe error prone polymerases and the process of translesion synthesis (TLS). In regards to tumor…

A: Translesion synthesis (TLS) is a method of overcoming stopped replication in which particular…

Q: NCAM is a gene that is normally only transcribed in neurons. How could you induce (force) expression…

A: The Neural cell adhesion molecule (NCAM), also referred to as CD56, may be a homophilic binding…

Q: Which of the following defects in RAS would be tumorigenic? multiple answers    A. Deletion of…

A: Ras is a nucleotide binding protein that is responsible for cell division, growth and…

Q: E. coli has five genes that code for enzymes that make tryptophan. These genes are regulated by a…

A: Tryptophan operon found in E.coli and some other bacteria. It is group of genes which encodes for…

Q: c) You use chromatin immunoprecipitation to measure the location and amount of transcription factors…

A: Transcription factors are protein molecules which bind to specific DNA sequences to regulate gene…

Q: A mutation in the trp repressor gene that prevents the apo-repressor from binding the co-repressor…

A: Tryptophan operon Tryptophan operon is negatively regulated operon, it includes five gene, promotor,…

Q: Which of the following accurately describes the function of HOTAIR? O It is a long non-coding RNA…

A: Option 1 is the correct answer.

Q: A disease is caused by having no functional protein produced from the kip gene. An individual has…

A: Mutations are alterations in the DNA sequence of an organism. Small changes, such as adding or…

Q: (i) A repressor binds to RPA and an activator binds to RPC 1. [Select] 2. [Select] 3. [Select]

A: Gene expression, the process by which information from a gene is used to synthesize a functional…

Q: Three similar proteins are expressed in human tissues: HHT1 is expressed in the liver,  HHT2 is…

A: Introduction Proteins are huge biomolecules and macromolecules that are made up of one or more long…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.