To identify transcriptional regulatory elements, reporters containing 100 base pairs ofPromoter Region DNA were tested in an expression system, where higher numbersindicate higher expression levels. The black boxes indicate the region mutated in eachreporter. Based on these results, where are the positive and negative regulatoryelements located?Reporter geneexpressionMutantPromoter Regionnumber-100-80-60-40-20+15104Positive: -40 to -10; Negative: -80 to -40Positive: -40 to -20; Negative: -70 to -60Positive: -70 to -60; Negative: -40 to -20Positive: -20 to -10; Negative: -80 to -70There are no positive or negative elements in the Promoter Region

Transcriptional regulatory elements are the genetic segments that act as the binding sites for…

Answered: To identify transcriptional regulatory…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A mutation in which of the following would still allow RNA polymerase to bind to promoters but would prevent the formation of an open complex? Question 4 options: TFIIB Mediator TFIIH TFIID TBP
S-Cdk is likely to be a [Select] The inhibitor of Ras is likely to be a [Select] The MAP kinase is likely to be a [Select] Q Search 14:31 Protein X is a transcription factor that normally activates the expression of genes needed for S- phase. The activator of protein X is likely to be a [Select] is 4 ******* > 21**113 ***** ****** V f6 99+ 1- Il app.honorlock.com is sharing your screen. **** 1 a hp Stop sha
You are studying how the expression of Gene F is regulated. Initially, you identified three potential regulatory elements in the regulatory region upstream of Gene F's transcription start site. To study these potential regulatory elements, you created three different mutants with a different potential regulatory element mutated in each. The data below shows qPCR data for expression of Gene F in Wild-type (Control) samples, each of the three different mutants (Mutant 1-3), and a negative control (No DNA Control). Were you able to identify a regulatory element that binds a transcriptional activator? If so, explain how you determined that and which mutant it is. Make sure to reference specific data from the image and your knowledge of the molecular biology involved in regulating transcription. The dotted line represents our measurement threshold. Fluorescence N Wild-Type Control Mutant 1 Mutant 2 Mutant 3 No DNA Control 10 10 20 30 Cycle
In eukaryotes, transcription factor activators bind to their enhancer elements and interact with RNA polymerase only through mediators. O True O False
Promoters and enhancers are important regulatory sequences for many eukaryotic protein-coding genes. Which of the following characteristics do these different sequences have in common? 1.Understood by the cell as double-stranded DNA. 2.Mutations in their sequence may result in increased gene expression. 3.Contain binding sites for proteins. 4.Must be upstream of the gene they influence. 1 and 3 4 only 2 and 4 1,2 and 3
The bacterial operon (a set of co-expressed genes) shown below contains one promoter (green), two terminators (stop signs), and three genes (coloured boxes). If 100 units of the red gene product are expressed, how much of each of the blue gene products are made? 100 200 It depends
Please answer asap
6 of 16 Which gene would most likely be under inducible expression control? O A gene that encodes for a protein required for the electron transport pathway O A gene that encodes for a protein critical for assembly of the bacterial cell wall A gene that encodes for a subunit of the RNA polymerase holoenzyme A gene that encodes for a protein that is produced in response to oxidative stress A gene that encodes for a component of a bacterial flagellar assembly
Part A You were sequencing a region of 200 kb in Effrenium voratum (a member of the family Symbiodiniaceae) and you found two genes, an insulator, and two enhancers, as shown below. Gene A Promoter gene A Enhancer I Enhancer I| Insulator Promoter gene b Gene B Q: What would happen if you do some gene editing and you move the insulator between both enhancers? The newly positioned insulator allows enhancer II to activate gene B transcription The newly positioned insulator allows enhancer I to hyperactivate gene B transcription The new positioning of the insulator prevents gene A expression It will be unaltered The newly positioned insulator prevents enhancer II from stimulating the transcription of gene B
amount of protein / amount of mRNA 12 mm / ↑ A B time (minutes) 20 Bacteria were grown in culture. At various time points, substances were added or removed from the culture medium. To assay the effects of the substances on operon gene expression, samples of bacteria were collected at one-minute intervals and levels of specific proteins and mRNAs were determined. If the operon under investigation is a repressible operon, which arrow indicates the addition of a molecule that binds the repressor protein?
Match whether the protein binds directly or indirectly (assuming it does) to DNA and whether to the enhancer or promoter. Items may be matched to multiple choices and not all choices will be used. 1. Directly to enhancer Repressors 2. Directly to promoter Activators 3. Indirectly to enhancer TBP associated factors 4. Indirectly to promoter >
Which of the following parts of the eukaryotic promoter are bound by general transcription factors? Group of answer choices A promoter proximal elements B enhancer elements C specific promoter elements D the core promoter E the distal promoter

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