Please note that Hrb27C is a gene located on chromosome 2L and FRT40A refers to an FRT sequence inserted at the 40A (cytological location). Tubulin (Tub) refers to a promoter that can drive target gene expression in all the cell types. Gal80 is an inhibitor of Gal4 that blocks Gal4’s ability to activate transcription. Gal4 will be expressed but it will not activate GFP in any of the somatic cells without heat shock. After heat shock, FLP recombinase will be induced, which will induce mitotic recombination at the FRT40A location, resulting in the generation of clones of cells with homozygous hrb27c mutation and homozygous P{Tub-Gal80} insertion. After heat shock, FLP recombinase will be induced, which will induce mitotic recombination at the FRT40A location. However, hrb27c mutant clone will not be generated because FRT40 and hrb27c mutation are located on different chromosome arms. After heat shock, the hrb27c mutant clones generated can be identified by the absence of GFP expression. After heat shock, GFP-expressing cells can be observed and these cells correspond to the hrb27c mutant clones.

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Please note that Hrb27C is a gene located on chromosome 2L and FRT40A refers to an FRT sequence inserted at the 40A (cytological location). Tubulin (Tub) refers to a promoter that can drive target gene expression in all the cell types. Gal80 is an inhibitor of Gal4 that blocks Gal4’s ability to activate transcription.

Gal4 will be expressed but it will not activate GFP in any of the somatic cells without heat shock.

After heat shock, FLP recombinase will be induced, which will induce mitotic recombination at the FRT40A location, resulting in the generation of clones of cells with homozygous hrb27c mutation and homozygous P{Tub-Gal80} insertion.

After heat shock, FLP recombinase will be induced, which will induce mitotic recombination at the FRT40A location. However, hrb27c mutant clone will not be generated because FRT40 and hrb27c mutation are located on different chromosome arms.

After heat shock, the hrb27c mutant clones generated can be identified by the absence of GFP expression.

After heat shock, GFP-expressing cells can be observed and these cells correspond to the hrb27c mutant clones.

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