MRNA 20 ORI 40 - FTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGHAATATOGGGATGCACTATC AAGCTCGAGAGCAGCAGCTCTATGCGCTACTATAATGACCANTATACECCTACGTGATAG 60 promoter RNA polymerase 4a. Was this micrograph taken of a sample prepared from human cells or prokaryotic cells? How do you know? 4b. What is the sequence of the first 10 nucleotides of the transcript of this gene? 3' 4c. What are the first 5 amino acids encoded by this gene? available at the beginning of this exam. ) Note - there is a codon table N'. 4d. Will translation stop at the UAA which begins at position 41? Explain your logic ribosome

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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You continue to study the expression of the hexose kinase gene and capture the following electron
micrograph of the gene being expressed.
MRNA
1
20
ORI
40
60
TTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGTAATATGGGGATGCACTATC
AAGCTCGAGAGCAСCAGCTСТАТGCGСТАСТАТААТGAССАКТАТАСССТАCGTGATAG
5"
3'
RNA
promoter
polymerase
4a. Was this micrograph taken of a sample prepared from human cells or prokaryotic cells? How do
you know?
4b. What is the sequence of the first 10 nucleotides of the transcript of this gene?
5'
3'
---
4c. What are the first 5 amino acids encoded by this gene?
)Note - there is a codon table
available at the beginning of this exam.
N'
C'
4d. Will translation stop at the UAA which begins at position 41? Explain your logic
4e. You also study the expression of 3 different mutants for this gene. For each mutant answer the
following:
Does this mutation change the sequence of the protein produced? Why or why not?
If it does change the sequence of protein be sure to write out the new sequence.
o If it does not change the protein sequence, what effect (if any) would you expect it
to have on expression of the gene?
1
20
ORI
40
60
5'.TTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGTAATATGGGGATGCACTAT.3'
3'...АAGCTCGAGAGCAGCAGCTCТАТСCGCTАСТАТААTGACCATTAТАССССТАСGTGATAG..5'
promoter
i. Mutant A has a single base pair substitution with the TIA being replaced with C/G base pair at
position 35 (position denoted by the * in the sequence above).
ii. Mutant B has a 2 GIC pairs inserted between position 19 and 20 (position denoted by the ^ in
the sequence above).
ii. Mutant C has had the first 5 base pairs deleted (position 1-5). Does this mutation change the
sequence of the protein produced? Why or why not? If it does change the sequence of protein
be sure to write out the new sequence. If it does not change the protein sequence, what effect
(if any) would you expect it to have on expression of the gene?
ribosome
Transcribed Image Text:You continue to study the expression of the hexose kinase gene and capture the following electron micrograph of the gene being expressed. MRNA 1 20 ORI 40 60 TTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGTAATATGGGGATGCACTATC AAGCTCGAGAGCAСCAGCTСТАТGCGСТАСТАТААТGAССАКТАТАСССТАCGTGATAG 5" 3' RNA promoter polymerase 4a. Was this micrograph taken of a sample prepared from human cells or prokaryotic cells? How do you know? 4b. What is the sequence of the first 10 nucleotides of the transcript of this gene? 5' 3' --- 4c. What are the first 5 amino acids encoded by this gene? )Note - there is a codon table available at the beginning of this exam. N' C' 4d. Will translation stop at the UAA which begins at position 41? Explain your logic 4e. You also study the expression of 3 different mutants for this gene. For each mutant answer the following: Does this mutation change the sequence of the protein produced? Why or why not? If it does change the sequence of protein be sure to write out the new sequence. o If it does not change the protein sequence, what effect (if any) would you expect it to have on expression of the gene? 1 20 ORI 40 60 5'.TTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGTAATATGGGGATGCACTAT.3' 3'...АAGCTCGAGAGCAGCAGCTCТАТСCGCTАСТАТААTGACCATTAТАССССТАСGTGATAG..5' promoter i. Mutant A has a single base pair substitution with the TIA being replaced with C/G base pair at position 35 (position denoted by the * in the sequence above). ii. Mutant B has a 2 GIC pairs inserted between position 19 and 20 (position denoted by the ^ in the sequence above). ii. Mutant C has had the first 5 base pairs deleted (position 1-5). Does this mutation change the sequence of the protein produced? Why or why not? If it does change the sequence of protein be sure to write out the new sequence. If it does not change the protein sequence, what effect (if any) would you expect it to have on expression of the gene? ribosome
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