Help me figure out how I should interpret and read these graphs. We are looking to see whether the m6a acceptor mutant will have an impact on splicing. Just need a detail breakdown thanks. I am presenting at journal club and need help interpreting will the goals in mind. We are currently conducting experiments to understand how the splicing of circE7 relates to the splicing of linear E6*I.  Goal is to determine whether the mutation impact splicing. Trying to understand whether m6A will impact splicing. Sm should impact splicing ratio. My PI stated that the results show that it inhibited linear splicing and promoted backsplicing. I need a detailed explanation for the entirety so I can understand

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Help me figure out how I should interpret and read these graphs. We are looking to see whether the m6a acceptor mutant will have an impact on splicing. Just need a detail breakdown thanks. I am presenting at journal club and need help interpreting will the goals in mind.

We are currently conducting experiments to understand how the splicing of circE7 relates to the splicing of linear E6*I. 

Goal is to determine whether the mutation impact splicing.

Trying to understand whether m6A will impact splicing.

Sm should impact splicing ratio.

My PI stated that the results show that it inhibited linear splicing and promoted backsplicing. I need a detailed explanation for the entirety so I can understand

HAMANO'Y
Relative Band Intensity
60
293T-Splicing Reporter - Spliced
55
50-
45-
40-
+
35
•••
1
WT
of 1
Silencer mutant
÷
m6A acceptor mutant
Relative Band Intensity
HeLa - Splicing Reporter - Spliced
70-
65-
60
55
+
50
of 1
B
ㅎㅎ
WT
Silencer mutant
····
m6A acceptor mutant
Transcribed Image Text:HAMANO'Y Relative Band Intensity 60 293T-Splicing Reporter - Spliced 55 50- 45- 40- + 35 ••• 1 WT of 1 Silencer mutant ÷ m6A acceptor mutant Relative Band Intensity HeLa - Splicing Reporter - Spliced 70- 65- 60 55 + 50 of 1 B ㅎㅎ WT Silencer mutant ···· m6A acceptor mutant
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