Many human genetic diseases are caused by mutations that occur at splice sites. Propose some ways that mutations at the 5′ splice site, 3′ splice site, and branch point might disrupt splicing and alter the phenotype.

Q: O a base substitution in an intron 5' splice site O a frameshift in an exon O a missense in an exon…

A: The answer to the question is  A frameshift in exon

Q: Consider the Rho-dependent terminator sequence 5’CCCAGCCCGCCUAAUGAGCGGCCUUUUUUUU-3’. What affect…

A: The mutation is the change in the nucleotide sequence of the DNA. The point mutation is the mutation…

Q: In prokaryotes, the promoter contains a -35 and -10 region upstream of the transcription start site.…

A: Promoter is the sequence in DNA where RNA polymerase is recruited. This is present before the…

Q: In the splicing of group I introns shown, does the 5′ end of the intron have a phosphate group?…

A: Introns are the inert elements in the DNA. They are otherwise said to be non coding regions of DNA…

Q: Nonfunctional HexA protein is responsible for the autosomal recessive disease Tay Sachs. A patient…

A: Ans... a base substitution in an enhancer region

Q: Suppose that the diagram below represents the genomic organization of an enzyme involved in eye…

A: Transcription is the process by which  RNA transcript is formed from DNA. Various type of RNA are…

Q: With respect to an intron, which one of the following options would be a good way to identify the…

A: DNA of eukaryotic genes contain split structures where segments of coding sequences are separated by…

Q: YFP-SC35 encodes a splicing factor. You would expect this molecule to be localized in what…

A: The process of transcription occurs in the nucleus. By this process, mRNA gets synthesized from the…

Q: Explain the process of transcription in prokaryotes, including the following: promoter region, RNA…

A: The DNA is the genetic material that is passed from one generation to the next generation. It is…

Q: Some mutations in the trp 5′ UTR increase termination by the attenuator. Where might these mutations…

A: Tryptophan operon consists of a leader region located at the 5' terminal of mRNA called 5' UTR. The…

Q: Which of the following statements about the mechanism of splicing is correct? 1. A mutation in a 5'…

A: The mRNA that is produced by the process of transcription undergoes a modification process in order…

Q: Briefly describe two important functions of the 5’ methyl guanine cap on eukaryotic mRNAs.

A: In eukaryotes, the nascent RNA that is synthesized by RNA polymerase II is called hnRNA…

Q: Identify one mistake that was introduced in the following figure.  True or false? The first few…

A: Since it is the method through which DNA is utilized as a template to produce RNA, transcription is…

Q: 4) The fact that splicing is a strategy to optimize the use of the genome in eukaryotes is evidenced…

A: Splicing: The biological process of splicing converts a freshly synthesized pre-mRNA into an adult…

Q: 293T-Splicing Reporter - Spliced Relative Band Intensity 60 55- ** *** ······· 1: :: HeLa-Splicing…

A: The following information discusses the relationship between Splice Reporter mRNA and Relative Band…

Q: Consider the CT/CGRP example of alternative splicing. Which different types of alternative splicing…

A: Alternative splicing is a technique that allows messenger RNA (mRNA) to control the production of…

Q: start with four exons and show mutually exclusive splicing producing 1-2-4 and 1-3-4 spliced exons.…

A: Mutually exclusive splicing is a phenomenon in which different exons are alternatively spliced,…

Q: In the picture below (representing RNA transcription) match A-E to whether they are 5' or 3' ends. A…

A: The information recorded inside the DNA sequence of one or more genes is transcribed into a strand…

Q: the encoded protein is substantially shorter than normal, and the amino acid sequence near the end…

A: Introduction The mutation is defined as any kind of alteration in normal base sequences of DNA.…

Q: Which of the following concerning progeria are false? Choose all that apply.

A: 1. Regularly, Zmpste24 slices pre-lamin to eliminate 50 amino acids.Zmpste24 is a catalyst engaged…

Q: What is the meaning of the term consensus sequence? Give an example. Describe the locations of…

A: The consensus sequences are widely used in molecular biology. The genes are controlled by the…

Q: a) Indicate the chronological order the following RNA processing events by indicating a number (from…

A: Step 1 Here's the chronological order of the pre-mRNA processing events: Pre-mRNA processing…

Q: Explain how an α helix in a transcription factor protein is able to function as a recognition helix.

A: Transcription is the first step in central dogma of protein synthesis. It involves formation of…

Q: Exon E10 E15 ..... .... RNA (protein) N Give TWO explanations as to how widely separated parts of a…

A: Exon are basically the functional parts of a gene which code for a functional protein. On the other…

Q: Identify the type of point mutation on the following strands (transition or transversion):  a. 5’…

A: The mutation is the sudden deleterious effects in the DNA sequences, they can arise when the DNA is…

Q: In Figure 16-3a, what is the consequence of the new 5′splice site on the open reading frame? In…

A: A deletion mutation can occur only when a wrinkle forms on the DNA template strand and this further…

Q: Select all the possible mutation types for the following observed phenotype resulting from a…

A: Mutation: Normal DNA contains a particular sequence of DNA. If the sequence of DNA is changed due to…

Q: The following eukaryotic DNA sequence is a made up gene. It contains a promoter (green), a coding…

A: Most eukaryotic genomes are bigger and more complicated than prokaryotic genomes. Eukaryotic genomes…

Q: Explain how self splicing occurs. Don't specify the base sequence of 5 prime and 3 prime splice…

A: Self-splicing is discussed below

Q: In eukaryotes, why is the initial RNA transcript usually longer than the mature mRNA molecule?…

A: Transcription is the process by which messenger rna is made from DNA.  This process takes place…

Q: Explain why inserting 5 bp of DNA at the –50 position of a eukaryotic gene decreases the rate of RNA…

A: The process of transcription occurs in the nucleus, during which RNA molecule is synthesized from…

Q: How often would the conserved sequence upstream of the 3' splice site, CURAY (R= PURINE, Y=…

A: There are a total of four different ribonucleotide bases in the RNA (ribonucleic acid). The…

Q: The U1 splice donor site is critical for positioning of the U1snRNP for appropriate splicing. What…

A: U1 snRNP (Small Nuclear Ribonucleoprotein Particle):The U1 snRNP is a component of the spliceosome,…

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.