CH,он, H. н, Он он Guanosine Guanosino- binding site Intron Exon 1 Exon 2 3' 5' 3' 3' OH 5% 3' RNA

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
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In the splicing of group I introns shown, does the 5′ end of the intron have a phosphate group? Explain.

CH,он,
H.
н,
Он
он
Guanosine
Guanosino-
binding site
Intron
Exon 1
Exon 2
3'
5'
3'
3'
OH
5%
3'
RNA
Transcribed Image Text:CH,он, H. н, Он он Guanosine Guanosino- binding site Intron Exon 1 Exon 2 3' 5' 3' 3' OH 5% 3' RNA
Expert Solution
Step 1

Introns are the inert elements in the DNA. They are otherwise said to be non coding regions of DNA which does not transcribe for any protein. They are removed during RNA splicing mechanism.

Step 2

In cells, which carry out the mechanism of self splicing, a nucleotide base begins the process of splicing. The two types of introns which carry out self splicing are group I and group II introns.

The mode of action of group 1 intron is: In this mechanism, a free guanosine nucleotide binds to a site which leads to the cleavage of the RNA at the 3’  end of exon 1. The 5’ end of exon 2 is cleaved with the help of another guanosine residue. The 3’ and 5’ ends are then joined by a covalent bond.

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