Several experiments were conducted to obtain information about how the eukaryotic ribosome recognizes the AUG start codon. In one experiment, the gene that encodes the methionine initiator tRNA (tRNA,Met) was identified and changed. Specifically, the nucleotides that specify the anticodon on the tRNA,Met were mutated so that the anticodon in the tRNA was 5'-CCA-3'. When this mutated gene was introduced into a eukaryotic cell protein synthesis occurred, but the proteins produced were abnormal. Some of these proteins contained extra amino acids and others contained fewer amino acids than normal. Explain why some of the proteins produced contained extra amino acids while others contained fewer amino acids than normal?  e. If the same experiment had been conducted in bacterial cells, what results would you expect? Explain.

Human Anatomy & Physiology (11th Edition)
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Several experiments were conducted to obtain information about how the eukaryotic ribosome
recognizes the AUG start codon. In one experiment, the gene that encodes the methionine initiator
tRNA (tRNA,Met) was identified and changed. Specifically, the nucleotides that specify the anticodon
on the tRNA,Met were mutated so that the anticodon in the tRNA was 5'-CCA-3'. When this
mutated gene was introduced into a eukaryotic cell protein synthesis occurred, but the proteins
produced were abnormal. Some of these proteins contained extra amino acids and others contained
fewer amino acids than normal.

Explain why some of the proteins produced contained extra amino acids while others
contained fewer amino acids than normal? 
e. If the same experiment had been conducted in bacterial cells, what results would you
expect? Explain.

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