Several experiments were conducted to obtain information about how the eukaryotic ribosome recognizes the AUG start codon. In one experiment, the gene that encodes the methionine initiator tRNA (tRNA,Met) was identified and changed. Specifically, the nucleotides that specify the anticodon on the tRNA,Met were mutated so that the anticodon in the tRNA was 5'-CCA-3'. When this mutated gene was introduced into a eukaryotic cell protein synthesis occurred, but the proteins produced were abnormal. Some of these proteins contained extra amino acids and others contained fewer amino acids than normal. a.) What is the normal anticodon for tRNA,Met? Show how this normal anticodon would interact with the codon for methionine in the mRNA. b.) What codon does the mutant anticodon bind? What amino acid is incorporated into the polypeptide chain? Show the interaction between the mutant anticodon and the amino acid codon and finally, What do the results described above indicate about how the ribosome recognizes the starting point for translation in eukaryotic cells?
Several experiments were conducted to obtain information about how the eukaryotic ribosome
recognizes the AUG start codon. In one experiment, the gene that encodes the methionine initiator
tRNA (tRNA,Met) was identified and changed. Specifically, the
on the tRNA,Met were mutated so that the anticodon in the tRNA was 5'-CCA-3'. When this
mutated gene was introduced into a eukaryotic cell protein synthesis occurred, but the proteins
produced were abnormal. Some of these proteins contained extra amino acids and others contained
fewer amino acids than normal. a.) What is the normal anticodon for tRNA,Met? Show how this normal anticodon would interact with the codon for methionine in the mRNA. b.) What codon does the mutant anticodon bind? What amino acid is incorporated into the polypeptide chain? Show the interaction between the mutant anticodon and the amino acid codon and finally, What do the results described above indicate about how the ribosome recognizes the starting point for translation in eukaryotic cells?
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