Phenylalanine (Phe) is encoded by either UUU or UUC, while Tyrosine (Tyr) is encoded only by UAC and UAU. Given this, and given what you know about tRNA selection, will Phenylalanine be found in the resulting polypeptides? (Assume translation will happen even without a start codon; Also this is a bit tricky, so review the contents of the assay before answering.) Yes, in part because phenylalanine is also coded for by UUU Yes, because ribosomes select the amino acid regardless of the anticodon sequence Yes, for both of the reasons listed above. No, because it is no longer attached to the tRNA with the GAA anticodon

Human Anatomy & Physiology (11th Edition)
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Phenylalanine (Phe) is encoded by either UUU or UUC, while Tyrosine (Tyr) is encoded only by UAC and UAU. Given this, and given what you know about tRNA selection, will Phenylalanine be found in the resulting polypeptides? (Assume translation will happen even without a start codon; Also this is a bit tricky, so review the contents of the assay before answering.)

  • Yes, in part because phenylalanine is also coded for by UUU
  • Yes, because ribosomes select the amino acid regardless of the anticodon sequence
  • Yes, for both of the reasons listed above.
  • No, because it is no longer attached to the tRNA with the GAA anticodon
Imagine that you repeat the Benzer tRNA Selection experiment with modifications as follows:
1. Synthesize mRNA containing U's and C's only (poly-UC in random order).
2. Convert the amino acid Phenylalanine (Phe) on its tRNA to the amino acid Tyrosine (Tyr) as shown below.
3. Mix your poly-UC RNA, your artificial TRNA, and cell extract (contains ribosomes, amino acids, all normal
TRNAS, and the energy source for translation).
Transcribed Image Text:Imagine that you repeat the Benzer tRNA Selection experiment with modifications as follows: 1. Synthesize mRNA containing U's and C's only (poly-UC in random order). 2. Convert the amino acid Phenylalanine (Phe) on its tRNA to the amino acid Tyrosine (Tyr) as shown below. 3. Mix your poly-UC RNA, your artificial TRNA, and cell extract (contains ribosomes, amino acids, all normal TRNAS, and the energy source for translation).
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