Determine whether each event occurs during initiation, elongation, or termination.InitiationInitiator tRNA enters the P site.ElongationAnswer BankIn E. coli, EF-Tu delivers an aminoacyl-tRNA to the ribosome.In prokaryotes, the Shine-Dalgarno sequence pairs with rRNA.The ribosome has mRNA, an empty A site, and a deacylated tRNA in the P site.IF2 dissociates.In E. coli, EF-Tu hydrolyzes GTP.TerminationTranslocation occurs.

The process of translation converts the information carried by messenger RNA from DNA into a…

Answered: Determine whether each event occurs…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Which of the following characterize RNA polymerase Il transcriptional termination in eukaryotes? Endonuclease cleavage of the RNA transcript and 5' to 3' exonuclease activity a protein known as Ratl None of the provided answers, transcriptional termination occurs in prokaryotes. Recognition of the transcriptional stop codon by a release factor. Hairpin structure formation on the newly synthesized RNA molecule which disrupts the DNA-RNA hybrid at a poly-U RNA sequence Binding of the Rho protein.
The antibiotic kasugamycin blocks the binding of methionine to tRNA. From this information, you can conclude that kasugamycin prevents: transcription in prokaryotes mRNA-ribosome binding in eukaryotes the synthesis of ribosomes by rRNA in eukaryotes the initiation of the synthesis of a protein in prokaryotes e. RNA polymerase from binding to a promoter
Suppressor tRNA often carries a mutation that change the anti-codon to match the mutated codon. Propose two other examples, where mutation in a RNA could suppress the mutation in another RNA, one from eukaryotes and the other from prokaryotes. You must specify the names for the RNA or RNA elements and the process they play roles in RNA 1 RNA 2 process prokaryote eukaryote
A certain amber suppressor inserts glutamine in response to the amber codon. What is the most likely change in the anticodon of a tRNAGln that created this suppressor strain?
Identify the correct recognition site for ribosomal subunit binding during translation. the 30s subunit binds to the 7-methylguanosine cap at the 3’ end of eukaryotic mRNA the 60s subunit binds to the “Shine-Dalgarno” sequence at the 5’ end of eubacterial mRNA the 40s subunit binds to the 7-methylguanosine cap at the 5’ end of eukaryotic mRNA the 70s subunit binds to the “Shine-Dalgarno” sequence at the 5’ end of eukaryotic mRNA the 50s subunit binds to the 7-methylguanosine cap at the 3’ end of eubacterial mRNA
Introns are often very large and the cell has devoted mechanisms of eliminating them once they are excised from the pre-mRNA. Following intron excision, what specific ribonucleolytic enzymes or complexes contribute to eliminating the intron RNA immediately after it is excised from the pre-mRNA? Briefly describe the role of each step/enzyme and how it affects its RNA substrate
Match the following (Most appropriate combinations only): IF1 IF3 IRES Shine-Dalgarno sequence m7-G Cap aminoacyl tRNA synthetase proofreading elF4E [Choose ] Binds ribosome A site Translation initiation in eukaryotes Translation initiation in Bacteria binds mRNA cap in eukaryotes Binds ribosome E site Correct charging of a tRNA with amino acid Cap-independent translation initiation of some viral mRNAs [Choose ] [Choose ] [Choose ] [Choose ]
During translational elongation, all tRNA’s enter the ribosome at the A site and exit the ribosome from its E site. Select one:TrueFalse
In the absence of cladosporin, explain the initiation steps in the synthesis of lysyl-tRNA synthetase enzyme or protein in the bacterial cell, with the involvement of all initiation factors, each site in the ribosome, any important conserved sequence, subunits of ribosomes, initiation codon, charged tRNA containing what amino acid, whether it requires ATP or GTP
Explain what is meant by the coupling of transcription and translation in bacteria. Does coupling occur in bacterial and/or eukaryotic cells? Explain.
In eukaryotic transcription, which of the following is not included in the initiation of transcription? Group of answer choices RNA polymerase II TATA box Holoenzyme AAUAAA consensus sequence

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