9 Which of the following statements about transcription is not true? In both prokaryotes and eukaryotes, pre-mRNA is modified after transcription by adding a 5' cap and a 3' poly-A tail and splicing out introns leaving coding exons. b O During transcription only one DNA strand called the template strand is read and rewritten by RNA polymerase with the template strand read in the 3 to 5' direction and the mRNA transcript synthesized in the 5' to 3' direction. Save space O During initiation of transcription, RNA polymerase binds to the promoter region in the DNA, unwinds the DNA and binds together RNA nucleotides complementary to the template DNA strand. O During initiation of eukaryotic transcription, the promoter region contains a TATA box in which transcription factors bind promoting the binding of RNA polymerase Il forming the transcription initiation complex. O During termination of transcription in prokaryotes RNA polymerase stops transcribing at the terminator sequence and in eukaryotes RNA polymerase stops transcribing at the polyadenylation sequence. Which of the following statements about translation is not true? O During translation, mRNA triplet codons are translated into amino acids. Each codon specifies just one amino acid but the same amino acid can be specified by more than one codon. O During initiation of translation, the small ribosomal subunit binds with mRNA and the initiator tRNA in the P site. The small subunit moves along the mRNA until it reaches the start codon where tRNA binds with it. Initiation factors facilitate the binding of the large ribosomal subunit forming the translation initiation complex. O During elongation of translation, codons are recognized by the ribosome and an appropriate complementary tRNA comes into the T site. A peptide bond forms between the growing polypeptide and the amino acid on the tRNA in the T site. Translocation moves the empty tRNA into the E site where it leaves the ribosome and the tRNA holding the polypeptide moves into the P site, now the next codon can be read. O During termination of translation, a stop codon in the mRNA is reached by the ribosome and a release factor comes into the A site causing the translation initiation complex to dissociate. O Post-translational protein modifications may occur including polypeptide folding or splicing