Both strands are shown; the top strand reads 5' to 3' left to right, while the bottom strand reads 5' to 3' right to left. The nucleotides are numbered 1 to 100. NOTE: For this problem, transcription begins with and includes the red and underlined CIG (top strand/bottom strand) base pair and RNA polymerase proceeds from left to right along the DNA. 1 20 40 5'-GTGTCCGTCTAАТАТТGTGAGATGTТАТАТСССGCСGTCAАCАССАТСАА-3' --+-- --- --- --- --- 3'-САСAGGCAGATTATAACAСТСТАСААТАТAGGGCGGCAGTTCTGGTAGTТ-5' 60 80 100 5'-ACAGGATAATCGCCTGCTGGGGCAAAGGCGGTGAAGGTAAAGGTGTTGCC-3' --+----- +--- -+-- ---+--- ----+ 3'-тстссТАтТAGCGGACGAСССССTTTCСGССАСТТССАТТтССАСААСGG-5' a) Which strand is used as a template for transcription, the top or the bottom? b) Where would the promoter be relative to the start of transcription? c) What are the first 15 nucleotides of the resulting mRNA? Indicate the 5' and 3' ends of the mRNA. d) What are the first 5 amino acids translated from the resulting mRNA? Indicate the amino acids translated from the resulting mRNA. Indicate the amino (NH,) and carboxy (COO') termini of the protein

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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3. Shown below is a double-stranded bacterial (E. coli) DNA sequence coding for a hypothetical protein.
Both strands are shown; the top strand reads 5' to 3' left to right, while the bottom strand reads 5' to 3'
right to left. The nucleotides are numbered 1 to 100. NOTE: For this problem, transcription begins with
and includes the red and underlined C/G (top strand/bottom strand) base pair and RNA polymerase
proceeds from left to right along the DNA.
1
20
40
5'-GTGTCCGTСТААТАТТGTGAGATGTТАТАТСССGCСGTCAАСАССАТСАА-3'
+-------
+-------
---------+
3'-САСAGGCAGATTATAACAСТСТАСААТАTAGGGCGGCAGTTCTGGTAGTТ-5'
60
80
100
5'-ACAGGATAATCGCCTGCTGGGGCAAAGGCGGTGAAGGTAAAGGTGTTGCC-3'
------+
3'-TGTCCTATTAGCGGACGACCCCGTTTCCGCCACTTCCATTTCCACAACGG-5'
a) Which strand is used as a template for transcription, the top or the bottom?
b) Where would the promoter be relative to the start of transcription?
c) What are the first 15 nucleotides of the resulting mRNA? Indicate the 5' and 3'
ends of the mRNA.
d) What are the first 5 amino acids translated from the resulting mRNA? Indicate the
amino acids translated from the resulting mRNA. Indicate the amino (NH,*) and
carboxy (COO') termini of the protein
Transcribed Image Text:3. Shown below is a double-stranded bacterial (E. coli) DNA sequence coding for a hypothetical protein. Both strands are shown; the top strand reads 5' to 3' left to right, while the bottom strand reads 5' to 3' right to left. The nucleotides are numbered 1 to 100. NOTE: For this problem, transcription begins with and includes the red and underlined C/G (top strand/bottom strand) base pair and RNA polymerase proceeds from left to right along the DNA. 1 20 40 5'-GTGTCCGTСТААТАТТGTGAGATGTТАТАТСССGCСGTCAАСАССАТСАА-3' +------- +------- ---------+ 3'-САСAGGCAGATTATAACAСТСТАСААТАTAGGGCGGCAGTTCTGGTAGTТ-5' 60 80 100 5'-ACAGGATAATCGCCTGCTGGGGCAAAGGCGGTGAAGGTAAAGGTGTTGCC-3' ------+ 3'-TGTCCTATTAGCGGACGACCCCGTTTCCGCCACTTCCATTTCCACAACGG-5' a) Which strand is used as a template for transcription, the top or the bottom? b) Where would the promoter be relative to the start of transcription? c) What are the first 15 nucleotides of the resulting mRNA? Indicate the 5' and 3' ends of the mRNA. d) What are the first 5 amino acids translated from the resulting mRNA? Indicate the amino acids translated from the resulting mRNA. Indicate the amino (NH,*) and carboxy (COO') termini of the protein
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