1. A portion of the template strand of a DNA molecule that codes for the 5'-end of an mRNA has the following nucleotide sequence. Give the primary structure of the polypeptide (use 3-letter amino acid codes), beginning with the most common translation initiation codon, that will be specified by this portion of the gene. Be sure to label the ends of the polypeptide. 3'-TTTTACGGGAATTAGAGTCGCAGGATG-5'

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### DNA to Polypeptide Transcription Exercise

**Question 1:**  
A portion of the template strand of a DNA molecule that codes for the 5’-end of an mRNA has the following nucleotide sequence. Give the primary structure of the polypeptide (use 3-letter amino acid codes), beginning with the most common translation initiation codon, that will be specified by this portion of the gene. Be sure to label the ends of the polypeptide.

**Template DNA Strand:**  
3’-TTTTACGGGAATTAGAGTCGCAGGATG-5’

**Steps to Find the Polypeptide Sequence:**

1. **Determine the mRNA Sequence:**
   - Transcribe the template DNA strand into mRNA. The mRNA sequence will be complementary to the template DNA and in the 5' to 3' direction.
     - DNA: 3’-TTTTACGGGAATTAGAGTCGCAGGATG-5’
     - mRNA: 5’-AAAUGCCCUUAAUCUCAGCGUCCUAC-3’

2. **Identify the Codons in the mRNA:**
   - Break the mRNA sequence into codons (sets of three nucleotides).
     - mRNA: 5’-AAA UGC CCU UAA UCU CAG CGU CCU AC-3’

3. **Translate the Codons to Amino Acids:**
   - Use the genetic code to convert each codon into its corresponding amino acid.
     - AUG: Methionine (Met) – Start codon
     - CCU: Proline (Pro)
     - UAA: Stop codon (translation stops here)

   - The resulting polypeptide sequence is:
     - **Polypeptide:** Met-Pro

**Final Answer:**

- **Polypeptide Sequence:** Met-Pro (NH2-Met-Pro-COOH)

Remember, translation begins at the start codon (AUG) and ends at a stop codon (UAA, UAG, or UGA). Thus, only the codons between these are translated into the polypeptide sequence.
Transcribed Image Text:### DNA to Polypeptide Transcription Exercise **Question 1:** A portion of the template strand of a DNA molecule that codes for the 5’-end of an mRNA has the following nucleotide sequence. Give the primary structure of the polypeptide (use 3-letter amino acid codes), beginning with the most common translation initiation codon, that will be specified by this portion of the gene. Be sure to label the ends of the polypeptide. **Template DNA Strand:** 3’-TTTTACGGGAATTAGAGTCGCAGGATG-5’ **Steps to Find the Polypeptide Sequence:** 1. **Determine the mRNA Sequence:** - Transcribe the template DNA strand into mRNA. The mRNA sequence will be complementary to the template DNA and in the 5' to 3' direction. - DNA: 3’-TTTTACGGGAATTAGAGTCGCAGGATG-5’ - mRNA: 5’-AAAUGCCCUUAAUCUCAGCGUCCUAC-3’ 2. **Identify the Codons in the mRNA:** - Break the mRNA sequence into codons (sets of three nucleotides). - mRNA: 5’-AAA UGC CCU UAA UCU CAG CGU CCU AC-3’ 3. **Translate the Codons to Amino Acids:** - Use the genetic code to convert each codon into its corresponding amino acid. - AUG: Methionine (Met) – Start codon - CCU: Proline (Pro) - UAA: Stop codon (translation stops here) - The resulting polypeptide sequence is: - **Polypeptide:** Met-Pro **Final Answer:** - **Polypeptide Sequence:** Met-Pro (NH2-Met-Pro-COOH) Remember, translation begins at the start codon (AUG) and ends at a stop codon (UAA, UAG, or UGA). Thus, only the codons between these are translated into the polypeptide sequence.
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