7. A molecule of mRNA from bacteria is shown below: 5'- GUUUCCGAGGAGGCGUAUACAUGUCAGUGGGGGC... -3' The ribosome binding site (RBS) is underlined. i. What molecule specifically binds to the ribosome binding site? ii. Briefly explain why this binding is necessary for initiation of translation. During translation, which amino acid will be attached to he first tRNA that binds in the A site of the ribosome? Immediately following peptide bond formation, where will ne amino acid chain be located? ( ) A site of the ribosome I) P site of the ribosome () E site of the ribosome F11s1 b a S e C G บบบ 1 UUC UUGLEU CUU CUC CUA CUG AUU AUC AUA AUG U GUU GUC GUA GUG PHE LEU ILE MET or START VAL UCU UCC UCA UCG C CCU CCC CCA CCGJ ACU ACC ACA ACG GCU GCC GCA GCG Second base SER >PRO THR ALA A UAU 1 UAC UAA UAG CAU CAC CAA 1 CAG AAU AAC AAA 1 AAG TYR STOP HIS GLN ASN LYS GAU ASP GAC GAA GLU UGU CYS UGC UGA STOP UGG TRP G CGU CGC CGA CGGJ AGU AGC G AGA AGG GGU GGC GGA GGGJ Describe the events necessary for termination of translation and release of the polypeptide chain. ARG SER ARG DOAO DOAG GLY U SCAG UCAG Ub ca
7. A molecule of mRNA from bacteria is shown below: 5'- GUUUCCGAGGAGGCGUAUACAUGUCAGUGGGGGC... -3' The ribosome binding site (RBS) is underlined. i. What molecule specifically binds to the ribosome binding site? ii. Briefly explain why this binding is necessary for initiation of translation. During translation, which amino acid will be attached to he first tRNA that binds in the A site of the ribosome? Immediately following peptide bond formation, where will ne amino acid chain be located? ( ) A site of the ribosome I) P site of the ribosome () E site of the ribosome F11s1 b a S e C G บบบ 1 UUC UUGLEU CUU CUC CUA CUG AUU AUC AUA AUG U GUU GUC GUA GUG PHE LEU ILE MET or START VAL UCU UCC UCA UCG C CCU CCC CCA CCGJ ACU ACC ACA ACG GCU GCC GCA GCG Second base SER >PRO THR ALA A UAU 1 UAC UAA UAG CAU CAC CAA 1 CAG AAU AAC AAA 1 AAG TYR STOP HIS GLN ASN LYS GAU ASP GAC GAA GLU UGU CYS UGC UGA STOP UGG TRP G CGU CGC CGA CGGJ AGU AGC G AGA AGG GGU GGC GGA GGGJ Describe the events necessary for termination of translation and release of the polypeptide chain. ARG SER ARG DOAO DOAG GLY U SCAG UCAG Ub ca
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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
Transcribed Image Text:### mRNA Structure and Translation Process
**7. mRNA Analysis from Bacteria:**
A sequence of mRNA is presented as follows:
```
5′- GUUUCCGAGGAGGCGUAUAC AUG UCAGUGGGGGC… -3′
```
**a. Ribosome Binding Site:**
i. **Binding Molecule:**
The ribosome binding site (RBS), also known as the Shine-Dalgarno sequence in bacteria, is underlined. The specific molecule that binds to the RBS is the *small subunit of the ribosome*.
ii. **Importance of Binding:**
Binding to the RBS is crucial for the initiation of translation because it positions the ribosome correctly on the mRNA, allowing the start codon (AUG) to align with the ribosome's peptidyl (P) site for the initiation of protein synthesis.
**b. First Amino Acid in Translation:**
During translation, the amino acid that will be attached to the first tRNA binding in the **A site** of the ribosome is determined by the start codon (AUG). According to the genetic code chart, this codon codes for *Methionine (MET)*, which is also the start of most protein synthesis processes.
**c. Location of Amino Acid Chain Post-Peptide Bond Formation:**
Immediately following peptide bond formation, the amino acid chain will be located in the:
- ( ) A site of the ribosome
- (**X**) P site of the ribosome
- ( ) E site of the ribosome
**d. Termination of Translation:**
The termination of translation involves several steps:
1. **Stop Codon Recognition:** When the ribosome encounters a stop codon (UAG, UAA, UGA) on the mRNA sequence in the A site, translation ceases as there are no corresponding tRNA molecules.
2. **Release Factors:** These proteins bind to the stop codon, prompting the release of the polypeptide chain from the tRNA in the P site.
3. **Dissociation:** The entire translation complex, including the mRNA, tRNA, and ribosomal subunits, dissociates, concluding protein synthesis.
**Genetic Code Chart Explanation:**
The accompanying diagram is a standard genetic code chart used to translate nucleotide triplets (codons) into amino acids. Each intersection of the
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