Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
2. H. Gobind Khorana deciphered the genetic code by (1) chemically synthesizing DNA molecules of known sequence, (2) using this synthetic DNA as a template to produce RNA in a reaction involving RNA polymerase and dNTPs, then finally (3) using this RNA in an in vitro translation experiment with the ribosome and amino acyl-tRNA (aa-tRNA) building blocks to produce peptides. For each single-stranded deoxyribonucleotide sequence Khorana synthesized (shown below), a single polypeptide was produced in the in vitro translation step (3): (1) Synthetic DNA d(TTT)n d(CCC)n d(AAA)n d(GGG)n d(AAACCC)n d(AC)n d(ACA)n C. (2) RNA encoded by synthetic DNA (3) Peptide product composition polylysine polyglycine polyphenylalanine polyproline Equal F and G Equal C and V polycysteine a. Fill in the RNA sequences that were produced by RNA polymerase in column (2). b. When Khorana conducted this work, one looming question in the field was whether or not the genetic code is overlapping or non-overlapping. In a non-overlapping triplet code, each group of three bases in a sequence ABCDEF... specifies only one amino acid - ABC specifies the first, DEF the second, and so on - whereas, in a completely overlapping triplet code, ABC specifies the first, BCD the second, CDE the third, and so on. Based on the experimental results shown above, is the genetic code an overlapping triplet code or a non-overlapping one? Explain how the data shown led you to this conclusion. Based on the peptide products observed in column three, assign each amino acid produced shown to the codon that encodes its installation in peptides.

2. H. Gobind Khorana deciphered the genetic code by (1) chemically synthesizing DNA molecules of known sequence, (2) using this synthetic DNA as a template to produce RNA in a reaction involving RNA polymerase and dNTPs, then finally (3) using this RNA in an in vitro translation experiment with the ribosome and amino acyl-tRNA (aa-tRNA) building blocks to produce peptides. For each single-stranded deoxyribonucleotide sequence Khorana synthesized (shown below), a single polypeptide was produced in the in vitro translation step (3): (1) Synthetic DNA d(TTT)n d(CCC)n d(AAA)n d(GGG)n d(AAACCC)n d(AC)n d(ACA)n C. (2) RNA encoded by synthetic DNA (3) Peptide product composition polylysine polyglycine polyphenylalanine polyproline Equal F and G Equal C and V polycysteine a. Fill in the RNA sequences that were produced by RNA polymerase in column (2). b. When Khorana conducted this work, one looming question in the field was whether or not the genetic code is overlapping or non-overlapping. In a non-overlapping triplet code, each group of three bases in a sequence ABCDEF... specifies only one amino acid - ABC specifies the first, DEF the second, and so on - whereas, in a completely overlapping triplet code, ABC specifies the first, BCD the second, CDE the third, and so on. Based on the experimental results shown above, is the genetic code an overlapping triplet code or a non-overlapping one? Explain how the data shown led you to this conclusion. Based on the peptide products observed in column three, assign each amino acid produced shown to the codon that encodes its installation in peptides.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
2. H. Gobind Khorana deciphered the genetic code by (1) chemically synthesizing DNA molecules of known sequence, (2) using this synthetic DNA as a template to produce RNA in a reaction involving RNA polymerase and dNTPs, then finally (3) using this RNA in an in vitro translation experiment with the ribosome and amino acyl-tRNA (aa-tRNA) building blocks to produce peptides. For each single-stranded deoxyribonucleotide sequence Khorana synthesized (shown below), a single polypeptide was produced in the in vitro translation step (3): (1) Synthetic DNA d(TTT)n d(CCC)n d(AAA)n d(GGG)n d(AAACCC)n d(AC)n d(ACA)n a. (2) RNA encoded by synthetic DNA (3) Peptide product composition polylysine polyglycine polyphenylalanine polyproline Equal F and G Equal C and V polycysteine Fill in the RNA sequences that were produced by RNA polymerase in column (2). b. When Khorana conducted this work, one looming question in the field was whether or not the genetic code is overlapping or non-overlapping. In a non-overlapping triplet code, each group of three bases in a sequence ABCDEF... specifies only one amino acid - ABC specifies the first, DEF the second, and so on - whereas, in a completely overlapping triplet code, ABC specifies the first, BCD the second, CDE the third, and so on. Based on the experimental results shown above, is the genetic code an overlapping triplet code or a non-overlapping one? Explain how the data shown led you to this conclusion. c. Based on the peptide products observed in column three, assign each amino acid produced shown to the codon that encodes its installation in peptides.
Transcribed Image Text:2. H. Gobind Khorana deciphered the genetic code by (1) chemically synthesizing DNA molecules of known sequence, (2) using this synthetic DNA as a template to produce RNA in a reaction involving RNA polymerase and dNTPs, then finally (3) using this RNA in an in vitro translation experiment with the ribosome and amino acyl-tRNA (aa-tRNA) building blocks to produce peptides. For each single-stranded deoxyribonucleotide sequence Khorana synthesized (shown below), a single polypeptide was produced in the in vitro translation step (3): (1) Synthetic DNA d(TTT)n d(CCC)n d(AAA)n d(GGG)n d(AAACCC)n d(AC)n d(ACA)n a. (2) RNA encoded by synthetic DNA (3) Peptide product composition polylysine polyglycine polyphenylalanine polyproline Equal F and G Equal C and V polycysteine Fill in the RNA sequences that were produced by RNA polymerase in column (2). b. When Khorana conducted this work, one looming question in the field was whether or not the genetic code is overlapping or non-overlapping. In a non-overlapping triplet code, each group of three bases in a sequence ABCDEF... specifies only one amino acid - ABC specifies the first, DEF the second, and so on - whereas, in a completely overlapping triplet code, ABC specifies the first, BCD the second, CDE the third, and so on. Based on the experimental results shown above, is the genetic code an overlapping triplet code or a non-overlapping one? Explain how the data shown led you to this conclusion. c. Based on the peptide products observed in column three, assign each amino acid produced shown to the codon that encodes its installation in peptides.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 5 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS