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BamHI Haelll Both 10 kb 9 kb 8 kb 5 kb 2 kb 1 kb Figure 2 How long is the original DNA molecule?

BamHI Haelll Both 10 kb 9 kb 8 kb 5 kb 2 kb 1 kb Figure 2 How long is the original DNA molecule?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A circular DNA molecule is subjected to complete restriction digestion by (1) BamHI alone, (2) Haelll alone, and (3) both enzymes together. The DNA fragments are then separated using gel electrophoresis. Results are shown in Figure 2: BamHI Haelll Both 10 kb 9 kb 8 kb 5 kb 2 kb 1 kb Figure 2 (i) How long is the original DNA molecule? (ii) Does the longest BamHI fragment contain a Haelll restriction site? Explain your answer. (ii) The same restriction digestion experiment was repeated for BamHI on the same DNA molecule. However, the incubation period of the reaction was lengthened for additional 30 minutes. Figure 3 illustrates the results after gel electrophoresis. Why is the result different from the earlier gel? Discuss your answer. BamHI 10 kb 9 kb 8 kb 5 kb 2 kb 1 kb Figure 3
Transcribed Image Text:A circular DNA molecule is subjected to complete restriction digestion by (1) BamHI alone, (2) Haelll alone, and (3) both enzymes together. The DNA fragments are then separated using gel electrophoresis. Results are shown in Figure 2: BamHI Haelll Both 10 kb 9 kb 8 kb 5 kb 2 kb 1 kb Figure 2 (i) How long is the original DNA molecule? (ii) Does the longest BamHI fragment contain a Haelll restriction site? Explain your answer. (ii) The same restriction digestion experiment was repeated for BamHI on the same DNA molecule. However, the incubation period of the reaction was lengthened for additional 30 minutes. Figure 3 illustrates the results after gel electrophoresis. Why is the result different from the earlier gel? Discuss your answer. BamHI 10 kb 9 kb 8 kb 5 kb 2 kb 1 kb Figure 3
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