1. The nontemplate strand of a segment of double- helical DNA contains the sequence: (5')CGCTATAGCGTTTAG (3') (a) What is the mRNA base sequence that can be transcribed from this double helical DNA assuming you already have a ready RNA polymerase present and everything else needed for RNA transcription? Label the sequence ends and list what is required for RNA Pol to work. (b) What amino acid sequence could be coded by the MRNA in (a)'s answer assuming the AUG start codon is just upstream of the mRNA (not shown in the sequence provided)? Use table in Figure 27-7 of the Lehninger 7 th ed
1. The nontemplate strand of a segment of double- helical DNA contains the sequence: (5')CGCTATAGCGTTTAG (3') (a) What is the mRNA base sequence that can be transcribed from this double helical DNA assuming you already have a ready RNA polymerase present and everything else needed for RNA transcription? Label the sequence ends and list what is required for RNA Pol to work. (b) What amino acid sequence could be coded by the MRNA in (a)'s answer assuming the AUG start codon is just upstream of the mRNA (not shown in the sequence provided)? Use table in Figure 27-7 of the Lehninger 7 th ed
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Question
Transcribed Image Text:1. The nontemplate strand of a segment of double-
helical DNA contains the sequence:
(5')CGCTATAGCGTTTAG (3') (a) What is the MRNA
base sequence that can be transcribed from this
double helical DNA assuming you already have a ready
RNA polymerase present and everything else needed
for RNA transcription? Label the sequence ends and list
what is required for RNA Pol to work. (b) What amino
acid sequence could be coded by the MRNA in (a)'s
answer assuming the AUG start codon is just upstream
of the MRNA (not shown in the sequence provided)?
Use table in Figure 27-7 of the Lehninger 7 th ed
"Dictionary of amino acid code words in mRNAs" and
write the peptide according to standard convention
with abbreviations for the amino acids. (c) If the wrong
strand was transcribed and then translated from the
section of double stranded DNA shown above, what
would the resulting amino acid sequence be? What
would the mRNA sequence be? Explain your answer
and label all strands.
A.
First letter of codon (5' end)
Second letter
of codon
UUU Phe UCU Ser
UAU Tyr UGU Cys
UGC Cys
u UUC Phe
UCC Ser
UAC Tyr
UAA Stop UGA Stop
UAG Stop UGG Trp
UUA Leu
UCA Ser
UUG Leu UCG Ser
CUU Leu CCU Pro
c CUC Leu
CUA Leu
CUG Leu CCG Pro CAG Gln
CGU Arg
CGC Arg
CAA Gln CGA Arg
CGG Arg
CAU His
СсC Pro
ССА Pro
CAC His
AUU lle
AUC lle
AUA lle
ACU Thr AAU Asn AGU Ser
АСС Thr
ACA Thr AAA Lys AGA Arg
AAC Asn AGC Ser
AUG Met ACG Thr AAG Lys AGG Arg
GCU Ala
GCC Ala
GCA Ala GAA Glu GGA Gly
GCG Ala GAG Glu GGG Gly
GAU Asp GGU Gly
GAC Asp GGC Gly
GUU Val
G GUC Val
GUA Va
GUG Val
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