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Imagine that you are studying the promoters of genes in E. coli. You notice that genes that are expressed in different conditions have different -10 and -35 sequences in their promoters. The genes expressed in condition B have -35 sequences of TTGACA, and -10 sequences of TATAAT. The genes expressed in condition C have -35 sequences of CTGGTA, and -10 sequences of TTGCA. What regulatory system is allowing these genes to be expressed in different conditions? The Catabolic Activator Protein, CAP. When CAMP is present, CAP binds the -10 and -35 sequences in the promoters of the genes expressed in condition B, and when CAMP is not present, CAP binds and activates transcription of the genes expressed in condition C. LysR regulators - a LysR regulator binds and activates transcription of certain genes in condition B, and, when a co-inducer is present, in activates transcription of other genes in condition C. O Lacl binds to the -10 and -35 sequences, and it has a different binding affinity for the different sequences, so it will repress the B genes more than it represses the C genes. Sigma factors - the sigma factor for condition B helps initiate the transcription of the genes expressed in condition B, and the sigma factor for condition C helps initiate the transcription of the genes expressed in condition C.

Imagine that you are studying the promoters of genes in E. coli. You notice that genes that are expressed in different conditions have different -10 and -35 sequences in their promoters. The genes expressed in condition B have -35 sequences of TTGACA, and -10 sequences of TATAAT. The genes expressed in condition C have -35 sequences of CTGGTA, and -10 sequences of TTGCA. What regulatory system is allowing these genes to be expressed in different conditions? The Catabolic Activator Protein, CAP. When CAMP is present, CAP binds the -10 and -35 sequences in the promoters of the genes expressed in condition B, and when CAMP is not present, CAP binds and activates transcription of the genes expressed in condition C. LysR regulators - a LysR regulator binds and activates transcription of certain genes in condition B, and, when a co-inducer is present, in activates transcription of other genes in condition C. O Lacl binds to the -10 and -35 sequences, and it has a different binding affinity for the different sequences, so it will repress the B genes more than it represses the C genes. Sigma factors - the sigma factor for condition B helps initiate the transcription of the genes expressed in condition B, and the sigma factor for condition C helps initiate the transcription of the genes expressed in condition C.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Imagine that you are studying the promoters of genes in E. coli. You notice that genes that are expressed in different conditions have different -10 and -35 sequences in their promoters. The genes expressed in condition B have -35 sequences of TTGACA, and -10 sequences of TATAAT. The genes expressed in condition C have -35 sequences of CTGGTA, and -10 sequences of TTGCA. What regulatory system is allowing these genes to be expressed in different conditions? The Catabolic Activator Protein, CAP. When CAMP is present, CAP binds the -10 and -35 sequences in the promoters of the genes expressed in condition B, and when CAMP is not present, CAP binds and activates transcription of the genes expressed in condition C. LysR regulators - a LysR regulator binds and activates transcription of certain genes in condition B, and, when a co-inducer is present, in activates transcription of other genes in condition C. Lacl binds to the -10 and -35 sequences, and it has a different binding affinity for the different sequences, so it will repress the B genes more than it represses the C genes. Sigma factors - the sigma factor for condition B helps initiate the transcription of the genes expressed in condition B, and the sigma factor for condition C helps initiate the transcription of the genes expressed in condition C.
Transcribed Image Text:Imagine that you are studying the promoters of genes in E. coli. You notice that genes that are expressed in different conditions have different -10 and -35 sequences in their promoters. The genes expressed in condition B have -35 sequences of TTGACA, and -10 sequences of TATAAT. The genes expressed in condition C have -35 sequences of CTGGTA, and -10 sequences of TTGCA. What regulatory system is allowing these genes to be expressed in different conditions? The Catabolic Activator Protein, CAP. When CAMP is present, CAP binds the -10 and -35 sequences in the promoters of the genes expressed in condition B, and when CAMP is not present, CAP binds and activates transcription of the genes expressed in condition C. LysR regulators - a LysR regulator binds and activates transcription of certain genes in condition B, and, when a co-inducer is present, in activates transcription of other genes in condition C. Lacl binds to the -10 and -35 sequences, and it has a different binding affinity for the different sequences, so it will repress the B genes more than it represses the C genes. Sigma factors - the sigma factor for condition B helps initiate the transcription of the genes expressed in condition B, and the sigma factor for condition C helps initiate the transcription of the genes expressed in condition C.
Both a repressor and an activator are in control of the lac operon, this ensures that lacZ and lacY are only transcribed when glucose levels are low and lactose levels are high. If the lacl gene were deleted from E. coli, how would that affect transcription of lacZY in media with high levels of glucose and lactose? Transcription of lacZY would be increased compared to the wild-type. Transcription of lacZY would be unaffected Transcription of lacZY would be decreased compared to the wild-type. Transcription of lacZY would be completely blocked.
Transcribed Image Text:Both a repressor and an activator are in control of the lac operon, this ensures that lacZ and lacY are only transcribed when glucose levels are low and lactose levels are high. If the lacl gene were deleted from E. coli, how would that affect transcription of lacZY in media with high levels of glucose and lactose? Transcription of lacZY would be increased compared to the wild-type. Transcription of lacZY would be unaffected Transcription of lacZY would be decreased compared to the wild-type. Transcription of lacZY would be completely blocked.
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