You identify a new transcriptional regulator that binds a known sequence of DNA. You are able to make mutants of this regulator and you know the sequence to which it binds. List two characteristics of these mutants or the binding sequence itself that would indicate the regulator acts as a homomultimer.

Q: Consider the Rho-dependent terminator sequence 5’CCCAGCCCGCCUAAUGAGCGGCCUUUUUUUU-3’. What affect…

A: The mutation is the change in the nucleotide sequence of the DNA. The point mutation is the mutation…

Q: In prokaryotes, the promoter contains a -35 and -10 region upstream of the transcription start site.…

A: Promoter is the sequence in DNA where RNA polymerase is recruited. This is present before the…

Q: Propose a final method of gene regulation of the Up Lateoperon using an updated drawn figure of the…

A: An operon is a part of the DNA where a few genes are clustered together that are transcribed…

Q: A full-length eukaryotic gene is inserted into a bacterial chromosome. The gene contains a complete…

A: The eukaryotic DNA is composed of coding and non-coding sequences, almost 90% are non-coding…

Q: Give the result(s) of the experiment in "MicroRNAs Control De Novo DNA Methylation Through…

A: INTRODUCTION MicroRNAs (miRNAs) are a class of small, single-stranded non-coding RNAs that regulate…

Q: Ignoring transcriptional methods, name two other "levels" aka methods a cell can use to ultimately…

A: The process of regulating which genes in a cell's DNA are expressed (used to create a functional…

Q: 3′-->5′ Exonuclease activity allows DNA polymerase III (Pol III) to back-up and fix a mismatched…

A: Since there are multiple questions in this particular question, I'll answer the first one for you.…

Q: Consider a mutant of E. coli that has an inactivating mutation in the gene for adenylate cyclase…

A: Inactivating mutations, also known as loss-of-function mutations, cause the gene product to have…

Q: hich operons would be functional under these conditions?   What repressors would be made? (NOTE:…

A: Operon is a regulatory unit consisting of cluster of genes containing operator promoter and…

Q: A string of 8 uracil nucleotides in the mRNA is required for function of an intrinsic transcription…

A: DNA transmits information to proteins in a continuous flow. DNA controls this basic central dogma…

Q: You made four mutants for a promoter sequence in DNA and studied them for transcription. The results…

A: Promoter are the sequence present in the DNA. These allow the binding of transcription factor and…

Q: Suppose you want to study the transcription in vitro of one particular gene in a DNA molecule that…

A: Transcription is the first step in gene expression during which the nucleotide sequence of DNA is…

Q: Some mutations in the trp 5′ UTR increase termination by the attenuator. Where might these mutations…

A: Tryptophan operon consists of a leader region located at the 5' terminal of mRNA called 5' UTR. The…

Q: 1) The UR operon which is composed of sequences A, B, C, and D (which may or may not be genes of…

A: An operon may be considered as a group of certain genes which function together so as to regulate…

Q: The E. coli genome contains approximately 4639 kb. (a) How many copies of the 6-bp recognition…

A: E.coli is a type of bacteria that normally lives in the intestine. It is also found in the gut of…

Q: Mutation in the regulatory gene in the lac operon results in the formation of an inactive repressor.…

A: The lac operon is an operon, or group of genes with a single promoter.The genes in the operon encode…

Q: RNAP can access the lacZ promoter irrespective of whether CAP-CAMP is binding the CAP o True False

A: Lac operon is a set of structural genes regulated by a common promoter. It is found in bacteria.

Q: Consider a gene being transcribed at a constant rate k1 and being degraded with first  order…

A: The genetic information from protein synthesis is stored in DNA. This information is copied onto an…

Q: Under low tryptophanyl-tRNA (tRNAtrp), we expect the trp operon to be expressed because:   Question…

A: Trp operon functioning in the bacterial system Escherichia coli consists of a set of genes that…

Q: A particular mutation in the bacterial sigma factor allows this protein to bind RNA polymerase but…

A: Answer - Option A - It will prevent the transcription termination exerted by the Rho protein

Q: In bacteria, genes that are often used together are controlled by a single promoter. Explain why…

A: Gene regulation refers to the numerous processes that regulate which genes are expressed and the…

Q: The chart below is a position specific scoring matrix (PSSM, a logarithmic transformed matrix) for a…

A: Hi, Thanks For Your Question.  Q2. Answer : The Maximum Score That A Sequence Can Have With This…

Q: Many transcriptional activators are proteins with a DNA-binding domain (DBD) and an activation…

A: Introduction  Protein is the key biomolecule in the biological system, any important physiological…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.