Suppose you had isolated a new transcription factor and wanted to know which genes this protein might regulate. Is there any way that you could use a cDNA microarray of the type shown in the picture to approach this question?

Q: You are a genetic engineer that is working on transcriptional regulation in living organisms. In…

A: Genetic engineering is the use of genetic knowledge to artificially manipulate genes: It is one of…

Q: Consider the Rho-dependent terminator sequence 5’CCCAGCCCGCCUAAUGAGCGGCCUUUUUUUU-3’. What affect…

A: The mutation is the change in the nucleotide sequence of the DNA. The point mutation is the mutation…

Q: One of Dr. Peῆa’s doctoral students, Marisol, finds out there are multiple mutant forms of U…

A: Given Antibiotic-resistance is one of the gravest dangers to the world’s medical system. As a…

Q: UP elements Select an answer and submit. For keyboard navigation, use the up/down arrow keys to…

A: Answer. A promoter can be defined as the cis-acting, position-dependent DNA sequence necessary for…

Q: A full-length eukaryotic gene is inserted into a bacterial chromosome. The gene contains a complete…

A: The eukaryotic DNA is composed of coding and non-coding sequences, almost 90% are non-coding…

Q: Some prion-related diseases, such as familial fatal insomnia, areinherited. How would you expect the…

A: Familial fatal insomnia is a medical condition characterized by insomnia or trouble sleeping. This…

Q: You have discovered a new bHLH gene and would like to use ChIP-Seq to learn more about it's role in…

A: A basic Helix-Loop-Helix Domain (bHLH) is a very common domain found in proteins that act as…

Q: What is a consensus sequence? How similar to the consensus sequence would you expect the promoter of…

A: Introduction  An area of DNA upstream of a gene called a promoter, as it relates to genomics, is…

Q: For the following gene, which type of regulatory sequence has likely been deleted in mutant 1?…

A: Transcription is the mechanism by which an RNA copy of a gene sequence is produced. This clone,…

Q: During experimental RNAi, how does the researcher affect expression of a target gene? Group of…

A: Introduction: In the process of RNA interference(RNAi) double-stranded RNA molecule suppresses the…

Q: (Theme 5) AraC is a transcriptional activator that binds to the AraC binding site and is encoded by…

A: The introduction of arabinose into the bacterial culture serves as a trigger for the activation of…

Q: A graduate student is trying to create a more efficient in vitro transcription system. The students…

A: The sigma factor is essential for identifying and attaching to particular promoter sequences, which…

Q: Suppose you want to study the transcription in vitro of one particular gene in a DNA molecule that…

A: Transcription is the first step in gene expression during which the nucleotide sequence of DNA is…

Q: Numerous diseases are treated by injection of polypeptide growth factors, such as human growth…

A: Scientists have modified the human cDNA of the growth factor to possibly improve its expression in…

Q: You identify a new transcriptional regulator that binds a known sequence of DNA. You are able to…

A: Identify a new transcriptional regulator that binds a known sequence of DNA.Observe the behavior of…

Q: How many types of promoters are there?

A: Promoters are DNA sequences that determine where the gene transcription by RNA polymerase begins.…

Q: c) You use chromatin immunoprecipitation to measure the location and amount of transcription factors…

A: Transcription factors are protein molecules which bind to specific DNA sequences to regulate gene…

Q: Consider a gene being transcribed at a constant rate k1 and being degraded with first  order…

A: The genetic information from protein synthesis is stored in DNA. This information is copied onto an…

Q: The following diagram show what is required for an active promoter of a gene of interest, where:A1 =…

A: Based on the given diagram, here are the predictions:Chromatin conformation: EuchromatinMethylation…

Q: How to incorporate prokaryotic gene into eukaryotic cell to make an transgenic organism?In this case…

A: Transformation is a technique used to incorporate foreign DNA into a target organism. In this case,…

Q: Suppose that E. coli sustains a mutation in its gene for the lac operon repressor making the…

A: When lactose is not present in the medium operon does not transcribe because of the presence of lac…

Q: In bacteria, genes that are often used together are controlled by a single promoter. Explain why…

A: Gene regulation refers to the numerous processes that regulate which genes are expressed and the…

Q: ncer where the newly-discovered genes BR and AIN are known to play a role in the development of this…

A: Cancer results from the uncontrolled cell division giving rise to a lump of cells that may be…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.