Using bacteria that express double stranded RNA upon induction by IPTG, investigators have constructed an RNAi library that allows you to feed C. elegans bacteria that expresses dsRNA corresponding to a given gene to obtain progeny that have been compromised for this target mRNA. This was performed for every predicted gene in the animal. From this experiment, 1200 genes of the 19000 predicted genes tested caused sterility in adult animals. 200 of the 1200 candidates that cased sterility were of unknown function. Q4a) What can we conclude from this information? Q4b) What processes are the genes with unknown function likely involved in?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Using bacteria that express double stranded RNA upon induction by IPTG, investigators have constructed an RNAi library that allows you to feed C. elegans bacteria that expresses dsRNA corresponding to a given gene to obtain progeny that have been compromised for this target mRNA. This was performed for every predicted gene in the animal. From this experiment, 1200 genes of the 19000 predicted genes tested caused sterility in adult animals. 200 of the 1200 candidates that cased sterility were of unknown function.
Q4a) What can we conclude from this information?
Q4b) What processes are the genes with unknown function likely involved in?
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