Imagine the same cells as above that has 20,000 molecules of estrogen receptor at the start of your experiment. You treat the cell with alpha amanitin, but forgot to add cycloheximide. The steady state level of estrogen receptor mRNA transcripts in the cell is 200. Ecah transcript takes two minutes to be translated into estrogen receptor protein. Assuming none of the ER mRNAs in the cell decay during the course of the experiment, at your 1 hour time limit, how many ER proteins will be present in the cell treated with only alpha aminitin?
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The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Imagine the same cells as above that has 20,000 molecules of estrogen receptor at the start of your experiment. You treat the cell with alpha amanitin, but forgot to add cycloheximide. The steady state level of estrogen receptor mRNA transcripts in the cell is 200. Ecah transcript takes two minutes to be translated into estrogen receptor protein. Assuming none of the ER mRNAs in the cell decay during the course of the experiment, at your 1 hour time limit, how many ER proteins will be present in the cell treated with only alpha aminitin?
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