What can you say about relative gene expression for the gene of interest, normalized to that of the housekeeper, using these cycle threshold data? Assume amplification efficiency is 100%. Note that gene expression is a measure of MRNA, but is accomplished by first converting the mRNA to copyDNA (CDNA) using reverse transcriptase. Average Ct Treatment A Treatment B Gene of interest 17.5 18.5 Housekeeping gene 18 17 It is 2-fold more abundant in treatment A. It is 2-fold more abundant in treatment B.
What can you say about relative gene expression for the gene of interest, normalized to that of the housekeeper, using these cycle threshold data? Assume amplification efficiency is 100%. Note that gene expression is a measure of MRNA, but is accomplished by first converting the mRNA to copyDNA (CDNA) using reverse transcriptase. Average Ct Treatment A Treatment B Gene of interest 17.5 18.5 Housekeeping gene 18 17 It is 2-fold more abundant in treatment A. It is 2-fold more abundant in treatment B.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:What can you say about relative gene expression for the gene of interest, normalized to that of the housekeeper, using these cycle
threshold data? Ássume amplification efficiency is 100%. Note that gene expression is a measure of MRNA, but is accomplished by first
converting the MRNA to copyDNA (cDNA) using reverse transcriptase.
Average Ct
Treatment A
Treatment B
Gene of interest
17.5
18.5
Housekeeping
gene
18
17
It is 2-fold more abundant in treatment A.
O It is 2-fold more abundant in treatment B.
It is 4-fold more abundant in treatment A.
It is 4-fold more abundant in treatment B.
It is 5.65-fold more abundant in treatment A.
O It is 5.65-fold more abundant in treatment b.
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