Suppose you cultured yeast, that you had transformed with pMZ379 plus the AVT5 sgRNA, on a plate of YES agar plus salt ions. You then cultured untransformed yeast on a plate containing YES agar plus salt ions. Your results showed growth on both plates. Which of the following is the most reasonable interpretation of your results? O A) The untransformed yeast spontaneously mutated to have a Cas9 gene that could silence the AVT5 gene. O B) The yeast had a mutation in the AVT5 gene that made the protein non- functional. O C) The sgRNA gene correctly guided the Cas9 protein to the AVT5 gene to silence it in the transformed yeast. D) The conditions for the experiment were so exceptional, that they promoted growth in both the modified and unmodified yeast.

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Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.