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Can you please explain what is happening in this figure? Please address controls, experimental method, what conclusions can be drawn and why the data supports these conclusions, and anything else... This data is taken from this article https://www.nature.com/articles/nature13994 Thank you so much

Can you please explain what is happening in this figure? Please address controls, experimental method, what conclusions can be drawn and why the data supports these conclusions, and anything else... This data is taken from this article https://www.nature.com/articles/nature13994 Thank you so much

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Can you please explain what is happening in this figure? Please address controls, experimental method, what conclusions can be drawn and why the data supports these conclusions, and anything else... This data is taken from this article https://www.nature.com/articles/nature13994

Thank you so much

a STARR-seq construct ORF hkCP or dCP Enhancer ORF hkCP S2 STARR-seq 1,822 4,134 (32%) 3,586 pA site TCT TATA box MTE DPE Inr dCP S2 STARR-seq AAAAA d dCP S2 STARR-seq enrichment (log₂) 6 b -2 CG33936 Fold change <2x hkCP and dCP 2x-4x hkCP.2x-4x dCP .>4x hkCP •>4x dCP 0 4 hkCP S2 STARR-seq enrichment (log₂) CG33937 2 6 Enr. rep2 (log₂) CG18473 CG8129 Enr. rep2 (log₂) Fps85D- hkCP S2 STARR-seq hkCP S2 STARR-seq dCP S2 STARR-seq Enr. rep1 (log₂) dCP S2 STARR-seq Enr. rep1 (log₂) e Per cent positive per tested 40 20 0 Luciferase-pA site hkCP luciferase 100 80 60 Luciferase construct 121/24 1/24 hkCP enhancer Or85f 2/12 dCP CG34117 CG8132 enhancer CG81354 CG9356 RpL34b Enhancer candidate dCP luciferase 10/12 6/7 6/7 ||| hkCP DCP or are Si Figure 1 Distinct sets of enhancers activate transcription from the hkCP and dCP in S2 cells. a, STARR-seq set-up using the hkCP housekeeping (RpS12; purple) and dCP developmental core promoters (Drosophila synthetic core promoter (DSCP)¹¹; brown) b, Genome browser screenshot depicting STARR-seq tracks for both core promoters. c, Overlap of hkCP and dCP enhancers. d, hkCP versus dCP STARR-seq enrichments at enhancers (insets show enrichment for replicates (Enr. rep) 1 versus 2 for hkCP and dCP; dCP data reanalysed from ref. 12). e, hkCP, dCP or shared enhancers that activate luciferase (>1.5-fold and P<0.05 (one-sided t-test); n = 3; Extended Data Figs 3 and 5) from hkCP (purple) or dCP (brown; numbers show positive/tested).
Transcribed Image Text:a STARR-seq construct ORF hkCP or dCP Enhancer ORF hkCP S2 STARR-seq 1,822 4,134 (32%) 3,586 pA site TCT TATA box MTE DPE Inr dCP S2 STARR-seq AAAAA d dCP S2 STARR-seq enrichment (log₂) 6 b -2 CG33936 Fold change <2x hkCP and dCP 2x-4x hkCP.2x-4x dCP .>4x hkCP •>4x dCP 0 4 hkCP S2 STARR-seq enrichment (log₂) CG33937 2 6 Enr. rep2 (log₂) CG18473 CG8129 Enr. rep2 (log₂) Fps85D- hkCP S2 STARR-seq hkCP S2 STARR-seq dCP S2 STARR-seq Enr. rep1 (log₂) dCP S2 STARR-seq Enr. rep1 (log₂) e Per cent positive per tested 40 20 0 Luciferase-pA site hkCP luciferase 100 80 60 Luciferase construct 121/24 1/24 hkCP enhancer Or85f 2/12 dCP CG34117 CG8132 enhancer CG81354 CG9356 RpL34b Enhancer candidate dCP luciferase 10/12 6/7 6/7 ||| hkCP DCP or are Si Figure 1 Distinct sets of enhancers activate transcription from the hkCP and dCP in S2 cells. a, STARR-seq set-up using the hkCP housekeeping (RpS12; purple) and dCP developmental core promoters (Drosophila synthetic core promoter (DSCP)¹¹; brown) b, Genome browser screenshot depicting STARR-seq tracks for both core promoters. c, Overlap of hkCP and dCP enhancers. d, hkCP versus dCP STARR-seq enrichments at enhancers (insets show enrichment for replicates (Enr. rep) 1 versus 2 for hkCP and dCP; dCP data reanalysed from ref. 12). e, hkCP, dCP or shared enhancers that activate luciferase (>1.5-fold and P<0.05 (one-sided t-test); n = 3; Extended Data Figs 3 and 5) from hkCP (purple) or dCP (brown; numbers show positive/tested).
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