You have found a gene of interest in an animal in the rain forest which produces a unique protein that will blocks reproduction of the pathogen that now plagues the world. You wish to mass produce this protein using bacteria. Answer the following questions regarding the scenario above. Describe how you would remove the gene of interest out of the isolated animal cell. What type of vector, (delivery system), would be best used to get the gene into the bacteria? How would you insert the gene of interest into the vector? How would get the vector into the bacteria? Why would the bacteria express this gene even if it normally does not have use for this protein?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
- You have found a gene of interest in an animal in the rain forest which produces a unique protein that will blocks reproduction of the pathogen that now plagues the world. You wish to mass produce this protein using bacteria.
Answer the following questions regarding the scenario above.
- Describe how you would remove the gene of interest out of the isolated animal cell.
- What type of vector, (delivery system), would be best used to get the gene into the bacteria?
- How would you insert the gene of interest into the vector?
- How would get the vector into the bacteria?
- Why would the bacteria express this gene even if it normally does not have use for this protein?
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