Isolate the human gene that produces factor VIII. Generate CDNA of the factor VIII gene using reverse transcriptase. Insert the factor VIII CDNA into a bacterial vector near a promoter site. Transform the vector into an E. coli bacterium. The factor VIII protein is isolated from human tissues. E. coli expresses factor VIII. Answer Bank Isolate the mRNA of the factor VIII gene. Human DNA is introduced into the bacterial cell using a projectile gun and DNA-coated tungsten.
Isolate the human gene that produces factor VIII. Generate CDNA of the factor VIII gene using reverse transcriptase. Insert the factor VIII CDNA into a bacterial vector near a promoter site. Transform the vector into an E. coli bacterium. The factor VIII protein is isolated from human tissues. E. coli expresses factor VIII. Answer Bank Isolate the mRNA of the factor VIII gene. Human DNA is introduced into the bacterial cell using a projectile gun and DNA-coated tungsten.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Arrange the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor VIII. (Not all steps will be placed).
Transcribed Image Text:Isolate the human gene that produces factor VIII.
Generate CDNA of the factor VIII gene using reverse transcriptase.
Insert the factor VIII CDNA into a bacterial vector near a promoter site.
Transform the vector into an E. coli bacterium.
The factor VIII protein is isolated from human tissues.
E. coli expresses factor VIII.
Answer Bank
Isolate the mRNA of the factor VIII gene.
Human DNA is introduced into the bacterial cell using a projectile gun and DNA-coated tungsten.
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