As a scientist in Company X, please design an in vitro transcription system for RNA vaccine synthesis. Please include the components required in the system and potential condition (e.g. temperature).
As a scientist in Company X, please design an in vitro transcription system for RNA vaccine synthesis. Please include the components required in the system and potential condition (e.g. temperature).
Ribonucleic acid is a single-stranded polymeric molecule that has a backbone made up of alternating sugar i.e ribose and the phosphate group. Each sugar has 4 bases: Adenine, guanine, uracil and cytosine. RNA is essential for major biological processes like coding, decoding, regulation, and expression of genes.
The RNA is of many types:
mRNA- It is the messenger RNA that transfers nucleic acid information from one genome to protein through the process of translation.
tRNA-It is the transfer RNA that carries specific three-letter-coded amino acids to the translation site(ribosome) and allows them to assemble into the chain of the protein.
rRNA- It is the ribosomal RNA that binds proteins and enzymes in the cytoplasm to form ribosomes which is the site for protein synthesis.
snRNA- Small nuclear RNA in the synthesis of mature mRNA from primary RNA molecules.
Regulatory RNA- it helps in the regulation of gene expression.
tmRNA- Transfer-messenger RNA are found in bacteria and plastids that help in tagging proteins that lack stop codons for degradation.
RNA enzymes-This RNA enzymes are also called ribozymes that act as a bio- catalysts and have typical enzyme characteristics like the active site, the binding site for cofactor, and substrate.
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