### Explanation of the Image and its ContentThe image contains information about an experiment involving an immunoblot (also known as a Western blot) that illustrates the time-dependent effects of MG132, a proteasome inhibitor, on different proteins. The study is referenced from Wang et al., (2019), in the journal Oncogene.#### Immunoblot Details- **Samples**: Taken at different time points (0, 0.5, 1, 2, 4, 8, 12, 24 hours) after treatment with MG132.- **Proteins** detected: - **P53** - **P27** - **AGR2** - **GAPDH** (used as a loading control)Each lane on the blot represents a different time point after MG132 treatment. The intensity of the bands indicates the relative abundance of each protein over time.#### Questions and Analysis##### a) Effects of MG132 on Proteins:For the proteins listed (P53, P27, AGR2), students are invited to analyze the data provided by the immunoblot to determine:- The changes in protein levels over time in response to MG132 treatment.- Implications regarding the degradation process mediated by the proteasome for each protein.##### b) Assurance of Equal Gel Loading:An important methodological consideration is ensuring that observed differences in protein levels are due to biological effects rather than experimental error, such as unequal loading of samples. GAPDH serves as a control to verify even sample loading across lanes.By analyzing the uniformity of the GAPDH band across all lanes, one can infer the consistency of loading, ensuring that variations in the other proteins are due to the MG132 effect and not technical errors.

Answered: 5) The immunoblot shows probing of…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Related questions

Concept explainers

Question

### Explanation of the Image and its Content

The image contains information about an experiment involving an immunoblot (also known as a Western blot) that illustrates the time-dependent effects of MG132, a proteasome inhibitor, on different proteins. The study is referenced from Wang et al., (2019), in the journal Oncogene.

#### Immunoblot Details

- **Samples**: Taken at different time points (0, 0.5, 1, 2, 4, 8, 12, 24 hours) after treatment with MG132.
- **Proteins** detected:
- **P53**
- **P27**
- **AGR2**
- **GAPDH** (used as a loading control)

Each lane on the blot represents a different time point after MG132 treatment. The intensity of the bands indicates the relative abundance of each protein over time.

#### Questions and Analysis

##### a) Effects of MG132 on Proteins:

For the proteins listed (P53, P27, AGR2), students are invited to analyze the data provided by the immunoblot to determine:
- The changes in protein levels over time in response to MG132 treatment.
- Implications regarding the degradation process mediated by the proteasome for each protein.

##### b) Assurance of Equal Gel Loading:

An important methodological consideration is ensuring that observed differences in protein levels are due to biological effects rather than experimental error, such as unequal loading of samples. GAPDH serves as a control to verify even sample loading across lanes.

By analyzing the uniformity of the GAPDH band across all lanes, one can infer the consistency of loading, ensuring that variations in the other proteins are due to the MG132 effect and not technical errors.

Expert Solution

Step 1

MG-132 is a potent proteasome and calpain inhibitor. Research studies have shown that MG-132 prevents degradation of the proteasome.

Proteasome inhibitor MG132 interfered with the NFκB canonical pathway of TNFα-treated C2C12 cells by protecting IκBα from degradation and thus preventing the activation of NFκB transcription.

Ubiquitin is a small, 76-amino acid, regulatory protein that was discovered in 1975. It's present in all eukaryotic cells, directing the movement of important proteins in the cell, participating in both the synthesis of new proteins and the destruction of defective proteins.

MG132 reduced proteolysis by more than 50% and protein synthesis by 10–20% in the muscles of healthy rats.

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions