Answered: Immunology question: Using cytometry…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Immunology question:

Using cytometry to stain a lymph node sample from a patient having a bacterial infection with a green fluorescent labeled anti-MHC I antibody and a red fluorescent-labeled anti-B7 antibody, what are the cells that are in each quadrant of the cytometry plot?

Note that the upper left is high in red and no green, the upper right is high red and high green, the lower left is none of red nor green, lower right is only high green.

My personal understanding Is:

UL: None: since there are no cells express B7 in lymph nodes without being APC.

UR: Activated antigen-presenting cells

LL: None APC cells including T cells, FDC; pathogens, etc.

LR: Non-activated APC cells. (Question, does all cells express MHC I ? if that's the case there should be a lot more)

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