In the RPO7 mutant lane, what is your interpretation of the results? Transcription of the 30S rRNA is blocked Cleavage of the 235 and 5S rRNAs is blocked, but the 165 is fine Cleavage of the 165 and 5S rRNAs is blocked, but the 235 is fine Cleavage of 16S rRNA is enhanced
In the RPO7 mutant lane, what is your interpretation of the results? Transcription of the 30S rRNA is blocked Cleavage of the 235 and 5S rRNAs is blocked, but the 165 is fine Cleavage of the 165 and 5S rRNAs is blocked, but the 235 is fine Cleavage of 16S rRNA is enhanced
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You are examining the processing of rRNA in E. Coli using a Northern blot. Normally, a 30S rRNA transcript is transcribed and then processed (cleaved) into 23S, 16S, and 5S mature rRNAs. You suspect the gene RPO7 is involved, so you make a mutant strain of bacteria and compare it to a Wild-type (Wt) strain. You run RNA extracts from the two strains on a Northern blot and probe it with a radioactive probe that binds to all rRNA. You get the following results.
Transcribed Image Text:You are examining the processing of rRNA in E. Coli using a Northern blot. Normally, a 30S rRNA transcript is transcribed and then processed (cleaved) into 23S, 16S, and 5S
mature rRNAs. You suspect the gene RPO7 is involved, so you make a mutant strain of bacteria and compare it to a Wild-type (Wt) strain. You run RNA extracts from the two
strains on a Northern blot and probe it with a radioactive probe that binds to all rRNA. You get the following results.
RPO7
Wild-Type Mutant
| |
In the RPO7 mutant lane, what is your interpretation of the results?
Transcription of the 30S rRNA is blocked
Cleavage of the 23S and 5S rRNAs is blocked, but the 16S is fine
Cleavage of the 16S and 5S rRNAs is blocked, but the 23S is fine
Cleavage of 16S rRNA is enhanced
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