You are attempting to prepare a single gene knockout library using the pRL27 transposon system. You grow the donor E. coli in Luria broth containing both kanamycin and diaminopimelic acid (DAP) and your recipient Serratia rubidaea in plain Luria broth. You combine an equal ratio of donor and recipient cultures and plate the mixture onto Luria agar supplemented with DAP. After 24 hours incubation at 37°C, you create a cell slurry and plate the cells onto Luria agar aupplemented with kanamycin. After 24 hours incubation at 37°C, you find that no colonies grow. What best explains this outcome?  A. Failure to supplement media with DAP  B.  Failure to remove antiobiotic containing media  C.  Failure to incubate for a sufficient length of time  D. Failure to incubate at the appropiate temperature  E. Failure to use the proper mating mix ratio

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Q: You are employed in a gene therapy laboratory to test the effectiveness of a vector for correcting…

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Q: Shown below are the complementation test results involving 4 independently isolated lethal mutants…

A: The correct answer is option c. 1 and 4   Mutants 1 and 4 are in the same group here.  In the…

Q: You are counting plaques on your plaque assay plates made from serial dilutions of your high titer…

A: A serial dilution is any dilution in which the concentration decreases by the same dilutions in each…

Q: For a coding gene, purifying (negative) selection will result in more synonymous (silent) site…

A: Purifying selection is the most predominant form of natural selection or negative selection. As it…

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A: Given, conjugation occurs between an (a+d+g+) donor and an (a-d-g-) recipient.  Hence, parental…

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Q: You are employed in a gene therapy laboratory to test the effectiveness of a vector for correcting…

A: Introduction: CFTR stands for Cystic Fibrosis Transmembrane Conductance Regulator. It is a protein…

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