laboratory to test the effectiveness of a vector for correcting the DF508 –CFTR mutation. The vector uses CRISPR –CAS9 genome editing to correct the mutated gene in situ (CFTR-CRISPR). An Ussing chamber experiment was performed using nasal airway cells obtained from a patient with the DF508 CFTR mutation which were transformed with either a control vector consisting of an inert CRISPR-CAS-9 construct (CONTROL) or the active, gene correcting CRISPR CAS-9 construct (CFTR-CRISPR). The following transepithelial voltage values were obtained for the drug regimen shown under open circuit and current clamp (injected current of 10mA) conditions:
You are employed in a gene therapy laboratory to test the effectiveness of a vector for correcting the DF508 –CFTR mutation. The vector uses CRISPR –CAS9 genome editing to correct the mutated gene in situ (CFTR-CRISPR). An Ussing chamber experiment was performed using nasal airway cells obtained from a patient with the DF508 CFTR mutation which were transformed with either a control vector consisting of an inert CRISPR-CAS-9 construct (CONTROL) or the active, gene correcting CRISPR CAS-9 construct (CFTR-CRISPR). The following transepithelial voltage values were obtained for the drug regimen shown under open circuit and current clamp (injected current of 10mA) conditions:
|
CONTROL |
|
Baseline |
Amiloride |
Forskolin |
Inh172 |
|
Vte (mV) |
-78 |
-2 |
-4 |
-3 |
|
|
DVRt (mV) |
8 |
16 |
7.5 |
13 |
|
|
R (W.cm-2) |
800 |
1600 |
750 |
1300 |
|
|
I (mA.cm-2) |
98 |
1 |
5 |
2 |
|
|
CFTR-CRISPR |
Vte (mV) |
-38 |
-6 |
-23 |
-9 |
|
DVRt (mV) |
9 |
27 |
6 |
11 |
|
|
R (W.cm-2) |
900 |
2700 |
600 |
1100 |
|
|
I (mA.cm-2) |
42 |
2 |
38 |
8 |
What is the size of the current that flows through ENaC for both vectors and What is the size of the current that flows through CFTR for both vectors?
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