A eukaryotic gene is added to a mixture containing a restriction enzyme EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has a recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA Iligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin nutrient broth plus tetracycline nutrient broth plus ampicillin and tetracycline nutrient broth only (without antibiotics) Which media would allow growth of bacteria that did not take up any plasmids? O the nutrient broth only O the nutrient broth and the tetracycline broth O the ampicillin broth and the tetracycline broth O the nutrient broth and the ampicillin broth

Q: Colonies/ug of plasmid) based on these results? O about 140O O about 800 O about 4000 O about 400…

A: INTRODUCTION Transformation efficiency is that the efficiency by which cells can take up…

Q: Plasmid is  3.6kb large. It is digested with an enzyme that has restriction sites at  1,152 and…

A: Plasmids are circular DNA found in bacteria. They are extra chromosomal materials and are highly…

Q: recombinant plasmid

A: DNA is the material that carries all the information about how a living thing will look and…

Q: What is a restriction enzyme? What structure does it recognize?What type of chemical bond does it…

A: Werner Arber, a Swiss microbiologist, winner of 1978 Nobel Prize in Physiology, for his discovery…

Q: A researcher has a plasmid vector that has been cut with a restriction enzyme that cuts after the…

A: Restriction enzymes are also called "restriction endonucleases". They are a group of enzymes that…

Q: Using the restriction site for SalI, suggest another restriction enzyme that would cut at the same…

A: Isoschizomers are a set of enzymes that share the same recognition sequence in the restriction…

Q: A student wishes to transform some E. coli with a plasmid to “knock-out” (ie. “silence” or prevent)…

A: The transformation is the natural process in which the donor bacteria releases the free DNA in the…

Q: nion and burger lover, your friend would like to incorporate the cheems gene (“cheM”) from the rare…

A: Transformation is a process in which the original DNA is transformed by the addition of foreign DNA…

Q: A circular plasmid of 10,000 base pairs (bp) is digested with two restriction enzymes, A and B, to…

A: in agarose gel electrophoresis we separate DNA bands based on their size.

Q: Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the…

A: The type of restriction enzymes that cut double stranded DNA at a particular spot is known as…

Q: You generate several temperature sensitive mutant strains of bacteria. To study what genes might be…

A: Introduction :- Bacterial mutants frequently lose a growth characteristic (such as the inability to…

Q: What are the advantages of using a restriction enzyme whose recognitionsite is relatively rare? When…

A: Restriction enzymes or restriction endonucleases are enzymes capable of recognizing a specific…

Q: plate your cells on a master plate without any antibiotics.  After colonies appear, you pick and…

A: Recombinant DNA technology is helpful for different gene related experiments.

Q: This is a map of PBR322 plasmid Nid If a recombinant plasmid were obtained inserting DNA into the…

A: Ans: Recombinant DNA technology: The technique of cutting and pasting of DNA molecule with another…

Q: A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A: Introduction :- Recombinant DNA technology ( also known as genetic engineering) involves the use of…

Q: Construct a restriction map for Plasmid X to show the recognition sites for two commonly used…

A: A restriction map is a representation of known restriction sites in a DNA sequence. Restriction…

Q: A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for…

A: "Biotechnology" is the use of our knowledge of biological processes to the development of beneficial…

Q: A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A: A plasmid vector is a small, circular DNA molecule that is commonly used in molecular biology for…

Q: DNA can be cut at specific sequence motifs using enzymes called restriction endonucleases. You…

A: One of the essential tools of molecular biology and recombinant DNA technology is restriction…

Q: Why is it necessary to prepare positive and negative control reactions in restriction digestion of a…

A: In restriction digestion of a plasmid (circular DNA molecule), preparing positive and negative…

Q: The diagram below shows that of a plasmid containing genes that confers erythromycin (Erm®) and…

A: Plasmids are small circular double-stranded DNA molecules that store additional genetic information…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A:

Q: plasmid has been modified such that it carries the gene for green fluorescent protein (GFP) under…

A: GFP protein is found in the photoorgans of Aequorea Victoria a jelly fish. It is a 28 kDa protein.…

Q: plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A:

Q: A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for…

A: The plasmid vector with the inserted gene of interest is called a recombinant plasmid. The process…

Q: All of the following are true about Restriction endonucleases except fashion

A: DNA fragments are cleaved at particular recognition sites by some enzymes known as Restriction…

Q: The Ti plasmid, is often used for making transgenic plants. Where the plasmid is found?

A: Bacteria are microorganism that most commonly occur in the soil, air, water and in adverse…

Q: A researcher has a Trp− auxotrophic strain of E. coliwith a mutation in a single gene. To identify…

A: An auxotrophic mutant refers to the strain having a mutation that does not allow the strain to…

Q: In a transformation experiment, a sample of competent bacteria was mixed with a plasmid containing…

A: In a bacterial colony some bacteria are resistant to an antibiotic and some are susceptible.…

Q: Margaret has been given a plasmid containing her favorite gene, afg (Margaret’s favorite gene). The…

A: First, convert the amount of plasmid from micrograms (μg) to nanograms (ng) since the concentration…

Q: Bacterial systems serve as an excellent model to express proteins but has a disadvantage – what is…

A: Expression of genes: Gene expression is the process of a gene being translated into a functioning…

Q: Jane has isolated a mutant strain of yeast that forms red colonies instead of the normal white when…

A: Plasmids are little pieces of extrachromosomal DNA found in the cytoplasm that can multiply on their…

Q: -gene--- 5'-GTCGACAAGCTTAAGCCTAAGCTTTAGCGAAGCTTGTCGAC-3' Recognition sites Sall 5'-GTCGAC-3'…

A: Sticky ends are short single-stranded overhanging segments of one DNA strand after the Double DNA is…

Q: In an attempt to clone a gene into the TOL plasmid, it was observed that the restriction product of…

A: Restriction Enzymes or “Restriction endonucleases” are proteins that cut DNA at a specific base…

Q: true or false if a restriction enzyme recognizes the restriction site, 5' AACGTT3', and the enzyme…

A: 1. Recognition Site of the Restriction Enzyme:The recognition site of a restriction enzyme is the…

Q: You utilised two plasmids in this practical, pOTC and pOTC-Δ. Plasmids are often represented using…

A: Bacteria produce the restriction enzyme, a protein that cleaves DNA at certain locations.  The…

Q: An E. coli genomic DNA fragment is run out on a gel undigested (U), and then with two different…

A: Restriction enzymes are those enzyme which recognise a specific recognition site in DNA and cuts the…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: To terminate a reaction in which a restriction enzyme cleaves DNA, researchers often add high…

A: Restriction enzymes are endonucleases that identify and cleave at specific sequences within DNA.…

Q: In an restriction enzyme experiment where Eco RI and  Hind III are used . What would happen if a…

A: Introduction  An isolated bacterial protein known as a restriction enzyme cleaves DNA at…

Q: b. After molecular cloning and confirming you properly cloned stripes into the vector shown, you…

A: The goal of this study is to investigate the structure of the protein product STRIPES, which…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.