A eukaryotic gene is added to a mixture containing a restriction enzyme EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has a recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA Iligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin nutrient broth plus tetracycline nutrient broth plus ampicillin and tetracycline nutrient broth only (without antibiotics) Which media would allow growth of bacteria that did not take up any plasmids? O the nutrient broth only O the nutrient broth and the tetracycline broth O the ampicillin broth and the tetracycline broth O the nutrient broth and the ampicillin broth

Q: If you were to grow the E. coli transformed with pGLO plasmid for several generations on…

A: pGLO plasmid  contains GFP gene to enable learning on central dogma, gene expression and regulations…

Q: For the Escherichia coli lactose operon genotypes listed, + indicates a functional component, -…

A: Permit me to elaborate on these lac operon strains' genotypes and phenotypes:  1. lacZ+ lacZ+ lacY+…

Q: As a Master's student in a lab you are given a plasmid vector that has the lacZ operon system and a…

A: INTRODUCTION - Insert recombinant DNA into host cell is called as Plasmid vector. EXPLANATION - STEP…

Q: Using the restriction site for SalI, suggest another restriction enzyme that would cut at the same…

A: Isoschizomers are a set of enzymes that share the same recognition sequence in the restriction…

Q: Which of the delivery techniques mentioned in Table 2 can be used to best transfer recombinant…

A: * They are many techniques for delivering the recombinant plasmids into the recipient cells. *…

Q: A student wishes to transform some E. coli with a plasmid to “knock-out” (ie. “silence” or prevent)…

A: The transformation is the natural process in which the donor bacteria releases the free DNA in the…

Q: pZERO®-1. Some vectors are designed specifically for TA cloning while some are not. What is TA…

A: A vector is a living organism that can transmits an infectious agent from an infected animal to…

Q: nion and burger lover, your friend would like to incorporate the cheems gene (“cheM”) from the rare…

A: Transformation is a process in which the original DNA is transformed by the addition of foreign DNA…

Q: For pGLO plasmid that contains araC gene and Ampr gene, what are the names of their promoters and…

A: pGLO plasmid  pGLO plasmid is a 5371bp genetically engineered plasmid to create genetically modified…

Q: Liliana is preparing chemically competent cells for heat shock transformation from old batches of…

A: i) E. coli DH5alpha is suitable to propagate plasmid before protein expression. It is a versatile…

Q: Many resistance mechanisms are encoded on plasmids. These mechanisms are of great clinical…

A: BLAST is a bioinformatics tool that enables us to identify a gene if we know its DNA sequence or the…

Q: plate your cells on a master plate without any antibiotics.  After colonies appear, you pick and…

A: Recombinant DNA technology is helpful for different gene related experiments.

Q: A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A: Introduction :- Recombinant DNA technology ( also known as genetic engineering) involves the use of…

Q: The F plasmid encodes which two functions essential for conjugation?

A: Plasmid encodes the Synthesis of F pilus and several other proteins required for Conjugation. Plasma…

Q: For a molecular cloning experiment using the bacterial strain E. coli K12 is used to insert the gene…

A: Recombinant DNA technology uses enzymes and a variety of laboratory procedures to modify and isolate…

Q: Most bacteria which are used in molecular biology are ampicillin sensitive and recA. Plasmid usually…

A: Mostly ampicillin sensitive and recA- bacteria are used in molecular biology for the transformation.…

Q: A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for…

A: "Biotechnology" is the use of our knowledge of biological processes to the development of beneficial…

Q: position of the Start and Stop. The GFP and RFP genes shown do not have start

A: The Cre-Lox system is a widely used tool in genetic engineering for site-specific modifications in…

Q: A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A: A plasmid vector is a small, circular DNA molecule that is commonly used in molecular biology for…

Q: Why are nucleic acid probes necessary to lyse cells containing plasmids in order to detect the…

A: Cell lysis is frequently the very first step in most purification techniques for nucleic acids,…

Q: You want to express the protein of a newly discovered human gene using E. coli, so you clone the…

A: Protein tags are most frequently used to purify proteins for which no protein-specific antibody…

Q: When using a conventional plasmid cloning vector containing a b-galactosidase gene, it is possible…

A: The blue–white screen is a techinque that allows for the rapid and convenient detection of…

Q: Why is it necessary to prepare positive and negative control reactions in restriction digestion of a…

A: In restriction digestion of a plasmid (circular DNA molecule), preparing positive and negative…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A:

Q: plasmid has been modified such that it carries the gene for green fluorescent protein (GFP) under…

A: GFP protein is found in the photoorgans of Aequorea Victoria a jelly fish. It is a 28 kDa protein.…

Q: Explain the THREE (3) important regions of this plasmid that enable it to work efficiently as a…

A: Plasmid is a self replicating generally circular DNA molecule that can be used to carry a foreign…

Q: You want to produce mass amounts of oxytocin, a small peptide hormone produced by the pituitary…

A: The molecular cloning is a technique of production of transgenic organisms. Here one gene is…

Q: Once cloning is complete, a recombinant OXA-M290 protein can be produced in E. coli. Competent E.…

A: The addition of IPTG to E. coli BL21(DE3) cells containing the pET-28a-OXA-M290 plasmid induces the…

Q: EDTA weakens the cell wall by removing ions that help hold it together while glucose prevents…

A: EDTA disrupts the outer membrane (enzymes that degrade DNA) by inhibiting DNases and chelating…

Q: plasmid has a total size of 2686 bp. There is an EcoRI recognition site at position 439, BamHI…

A: Plasmid  It is an extra chromosomal piece of DNA in the cell.

Q: Why do restriction endonucleases not hydrolyze DNA from the organism that produces it?

A: Endonucleases are the enzymes, which help to cleave the DNA from inside that is in between the…

Q: All of the following are true about Restriction endonucleases except fashion

A: DNA fragments are cleaved at particular recognition sites by some enzymes known as Restriction…

Q: The Ti plasmid, is often used for making transgenic plants. Where the plasmid is found?

A: Bacteria are microorganism that most commonly occur in the soil, air, water and in adverse…

Q: The following DNA sequence has been identified and named ‘Gene Z’. It is thought that this sequence…

A: PCR is a polymerase chain reaction, this is a biotechnological process which is used to amplify the…

Q: In Figure 5-19, how many different bacterial species areshown as having contributed DNA to the…

A: A plasmid is an extrachromosomal circular DNA molecule found in the bacteria which is able to…

Q: A researcher has a Trp− auxotrophic strain of E. coliwith a mutation in a single gene. To identify…

A: An auxotrophic mutant refers to the strain having a mutation that does not allow the strain to…

Q: The human gene for hemophilia is on the X chromosome. Below is a pedigree from a family afflicted…

A: Hi, Thanks For Your Question.  Answer : Correct Option Is b (20%) Explanation :  P (III-7 Is…

Q: our plasn igest tub

A: Introduction: A plasmid is a small, circular, double-stranded DNA molecule that is separate from a…

Q: You are on a research project to study rice. Your want to use reverse transcription PCR to amplify…

A: PCR stands for Polymerase Chain Reaction. It is a laboratory technique used to amplify or create…

Q: A molecular geneticist hopes to find a gene in human liver cells that codes for an important…

A: Introduction :- A nucleic acid sequence is a series of bases denoted by a set of five letters that…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.