A plasmid has been modified such that it carries the gene for beta-galactosidase (lacZ) under the control of the lac promoter. Between the lac promoter and the lacZ gene is an empty multiple cloning site (MSC). This plasmid also carries ampicillin resistance. E. coli are transformed with this plasmid, and grown on nutrient agar containing ampicillin, IPTG, and the lactose analogue Xgal for 18 hours. Following incubation, the plates are examined. What will be the likely colour of the bacteria colonies?
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
A plasmid has been modified such that it carries the gene for beta-galactosidase (lacZ) under the control of the lac promoter. Between the lac promoter and the lacZ gene is an empty multiple cloning site (MSC). This plasmid also carries ampicillin resistance. E. coli are transformed with this plasmid, and grown on nutrient agar containing ampicillin, IPTG, and the lactose analogue Xgal for 18 hours. Following incubation, the plates are examined. What will be the likely colour of the bacteria colonies?
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