Salmonella enterica can grow on the lipid breakdown product ethanolamine as a sole carbon and nitrogen source. Ethanolamine is abundant in the mammalian gut, especially during inflammation. You hypothesize that S. enterica has genes encoding a pathway specifically required for growth on ethanolamine. Design a mutant hunt that would allow you to identify Salmonella genes necessary for growth on ethanolamine, and state: • the method of mutagenesis you will use (and why) • are you using a screen, a selection, and/or an enrichment to identify relevant mutants? • the independent and dependent variables • both positive and negative controls • potential outcomes of your experiment, and how you will interpret them

Q: 1. a)What would happen if the aminoacyl tRNA synthetase responsible for charging alanine tRNAs also…

A: The polymerization of amino acids according to the codons on mRNA to form proteins is known as…

Q: Suppose that E. coli sustains a mutation in its gene for the lac operon repressor such that the…

A: An operon is the unit of bacterial gene expression and regulation. The operon contains the…

Q: During your experiment you analysed only a few of the recombinant clones for the presence of the…

A: A Genomic library consists of the total genomic DNA of an organism. This DNA can be stored inside…

Q: A full-length eukaryotic gene is inserted into a bacterial chromosome. The gene contains a complete…

A: The eukaryotic DNA is composed of coding and non-coding sequences, almost 90% are non-coding…

Q: Most of the mutations that Yanofsky recovered were missense mutations. However, Yanofsky also…

A: Hypothesis is basically a proposed explanation for any naturally occurring phenomenon in the…

Q: 32. Which of the following is always present on the basolateral membrane of renal tubule cells? a.…

A: Na+/K+ ATPase transporter is always present on the basolateral membrane of renal tubule cells.

Q: From the result shown, what might be the histidine biosynthetic pathway (the order of enzymes)?

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Q: In site-directed mutagenesis experiments of an enzyme, scientists altered an aspartate residue to…

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Q: e previous problem you proposed a model for how this gene could be regulated. Suppose that you carry…

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Q: Question 6: What do you think is the composition of the medium in the master plate (minimal, minimal…

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A: Mutation is the process of abrupt changes in the nucleotide sequence or gene in the DNA. Mutation…

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A: Isocitrate lyase is an enzyme in the glycoxylate cycle that catalyzes the cleavage of isocitrate to…

Q: Transposon mutagenesis

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.