In the bacterium,Martian coli, it was discovered that the lac operon is positively regulated. Lactose, when present, binds to the regulatory protein A. The protein A-lactose complex then binds to the operator domain 0 and induces synthesis of lacZ+ and lacY+ MRNAS. a) What would be the most common phenotype of o- mutants in terms of lactose utilization? Explain your answer. b) What would be the most common phenotype of A- mutants? Explain your answer.

Q: A lac operon containing one mutation was cloned into a plasmid, which was introduced by…

A: the lac operon system present in bacteria

Q: In the lac operon system, do the inducers act at the transcription or translation level? Explain why…

A: The lactose operon (lac operon) consists of a regulatory gene (i-gene) and three structural genes -…

Q: If the above gene is one of the three structural genes of the lac operon that codes for the protein/…

A: The bacterium Ecoli possess a highly effective lactose Metabolism process . The lac operon is a…

Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…

A: The Lac operon contains genes responsible for the transportation and breakdown of lactose. These…

Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…

A: Operon acts as a single regulated unit having one or more structural genes, promoter gene, a…

Q: What would happen if the operator sequence of the lac operon contained a mutation that prevented the…

A: The Structure Of lac operon --Genes present in the lac operon specify proteins which help in the…

Q: Attardi et al. (1963) found that lactose operon mRNA synthesis in coli increased with the addition…

A: Both structural and regulatory genes are found in genomic DNA, and both encode products that act as…

Q: The trp operon, found in E. coli, is a group of genes that encode biosynthetic enzymes for the amino…

A: When tryptophan levels are low tryptophan operon  is induced to transcribe structural genes so that…

Q: The trpR gene encodes an allosteric protein that binds the amino acid tryptophan and regulates the…

A: Trp operon is also known as tryptophan operon. The tryptophan operon consists of structural genes,…

Q: a) In an experiment, you have changed a DNA sequence directly upstream from a start codon of an…

A: The question says that transcription is not affected but translation is. Therefore, some sequence…

Q: . Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of…

A: The bacterial transcription unit containing more than one gene is known as an operon. An operon has…

Q: Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of E.…

A: Since we only answer up to 3 sub-parts, we’ll answer the first 3. Please resubmit the question and…

Q: You have isolated two different mutants (reg1 and reg2) causing constitutive expression of the emu…

A: A constitutive mutation causes the continuous production of the gene product. It means in this…

Q: Which of the following lac operon genotypes would allow for functional versions of all the…

A: lac operon: - Inducible system - Both positive and negative regulation. - Involved in lactose…

Q: . Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of…

A: INTRODUCTION: Tryptophan (trp) operon for the synthesis of the amino acid tryptophan, is an example…

Q: You can determine the regulation of an unknown operon based on descriptions like those in the table…

A: Introduction  The expression of genes is highly regulated in both prokaryotes as well as in…

Q: Let’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively…

A: E.coli is a gram negative bacterium which thrives in the intestine of mammals. E.coli is beneficial…

Q: If an abnormal repressor protein could still bind allolactose, but thebinding of allolactose did not…

A: Deoxyribonucleic acid is a particle made out of two polynucleotide chains that loop around one…

Q: Which of the following mutations would result in the highest level of lac operon transcription in…

A:

Q: Which of these operons is most similar to the lac operon? Explain.

A: A group of genes that transcribe to produce one mRNA is called an operon. This operon is responsible…

Q: If CAP is bound to the Promoter of the Lac Operon and the repressor protein is not bound to the…

A: Operon is the prokaryotic gene regulatory system that controls the expression of polycistronic mRNA.…

Q: What is the major difference between the mechanism involved inattenuation and riboswitches and the…

A: Attenuation is a mechanism that controls gene expression in a bacterial operon and causes premature…

Q: The lac operon is strongly transcribed when which of the following sugars are present in the growth…

A: The lac operon is an operon or group of genes with a single promoter (transcribed as a single mRNA).

Q: The lactose operon in E. coli comprises two structural genes and three regulatory components.…

A: β-galactosidase activity Permease activity Genotypes Glycerol Lactose Glycerol Lactose A - + -…

Q: Mutations may have an effect on the expression of the lac operon and the trp operon. Would the…

A: Mutations are defined as the change in the sequence of DNA of an organism due to any environmental…

Q: The lac operon consists of regulatory regions such as the promoter as well as the structural genes…

A: Lac operon is an inducible type of operon system takes place in prokaryotes like E.coli in which…

Q: What experimental results would indicate that the mutation lacISlacIS is dominant to lacI+lacI+?…

A: The correct option is (e) i.e, In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a…

Q: What if there was a mutation in the operator region of the lac operon and the active repressor could…

A: We are answering first 2 questions. For the remaining question pls repost.

Q: a) In an experiment, you have changed a DNA sequence directly upstream from a start codon of an…

A: Introduction :- Operon model was proposed by Jacob and Monod in 1961. In this model , they explained…

Q: Let’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively…

A: Escherichia coli contains genes present in the lac operon that are activated in the absence of…

Q: Which of the following environmental conditions would be MOST likely to result in transcription of…

A: The transcription of the lac Oberon in an E.colicell is high levels of lactose and high levels of…

Q: Under what conditions would you expect to see high levels of expression of the structural genes in…

A: Please find your answer in step2:

Q: Consider the trp operon found in E. coli. You have discovered a mutant strain of E.coli that…

A: The genome of the prokaryotic organisms is polycistronic i.e. the genes are controlled by one…

Q: If the above gene is one of the three structural genes of the lac operon that codes for the protein/…

A: Introduction Lactose operon(lac operon) is a system which includes genes that are essential for…

Q: If a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or…

A: The lac operon or the lactose operon is a mechanism that is involved in the metabolism and transport…

Q: What are the effects of the following conditions on Lac operon of bacteria? Please do not forget to…

A: Lac operon is the inducible system of catabolic degradation of lactose. Lac operon is a combination…

Q: If glucose levels in the cell are high and lactose is available from the environment, what is the…

A: Bacteria such as E.coli has developed an outstanding system of genes that together regulate the…

Q: What is the function of cAMP in regulation of the lac operon? Group of answer choices it activates a…

A: The lac operon seems to be an inducible operon that uses lactose as a source of energy and is active…

Q: Which of the statements about the attenuator region of the trp operon is TRUE? Choose an answer…

A: It is located upstream of the operator.

Q: Attardi et. al (1963) found that lactose operon synthesis in E.coli increased with the addition of…

A: operon, a genetic regulatory system found in bacteria and their viruses that clusters genes coding…

Q: A marine bacterium is isolated and shown to contain an inducible operon whose genetic products…

A: An operon is defined as a genetic regulatory system. It is present mostly in bacteria and…

Q: Explain how the lac operon is regulated under the following conditions. Include the following terms…

A: Lactose can be broken down by E. coli bacteria, however, it is not their preferred fuel. They would…

Q: What is an operon ? a. A series of genes controlled by the same operator b. A series of genes on the…

A: Operons are the functioning unit of DNA that consists multiple genes grouped together with an…

Q: A mutant strain of E. coli produces β-galactosidase in both the presence and the absence of lactose.…

A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…

Q: Suppose that E. coli sustains a mutation in its gene for the lac operon repressor making the…

A: The lactose operon or lac operon is an inducible operon system under dual – positive and negative…

Q: What does the lac operon consist of? How is the operator switch turned on and off in the expression…

A: Lac operon is the operon or machinery for the transport and metabolism of lactose in Escherichia…

Q: If glucose is not available, but lactose is available from the environment, what is the status of…

A: Points to keep in mind related to Lac operon : 1.  Presence of Lactose inhibits repressor protein…

Q: . Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of…

A: The bacterial transcription unit containing more than one gene is known as an operon. An operon has…

Q: In addition to observing similarities to the lac operon, you also notice that this gene is regulated…

A: Transcriptional attenuation negatively regulates the expression of many operons in bacteria by…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.