In the bacterium,Martian coli, it was discovered that the lac operon is positively regulated. Lactose, when present, binds to the regulatory protein A. The protein A-lactose complex then binds to the operator domain 0 and induces synthesis of lacZ+ and lacY+ MRNAS. a) What would be the most common phenotype of o- mutants in terms of lactose utilization? Explain your answer. b) What would be the most common phenotype of A- mutants? Explain your answer.

Q: The map of the lac operon is shown below. Consider the following examples that include both haploids…

A: Lac operon is the segment of DNA which includes regulatory gene, structural gene, promoter gene and…

Q: 1) The logic of the Lac operon was deciphered by using bacterial lac-operon mutants. Crucial to the…

A: Lac operon is a genetic unit in bacteria that helps in the metabolism of lactose in E.coli and lac…

Q: Strain X-23 has a mutation in the lacI gene resulting in a lacI protein that cannot bind DNA.…

A: Gene regulation at the level of transcription in bacteria is achieved by the operon model. Operon…

Q: Given the following mutant and conditions, predict the expression of the lacZ gene (no expression,…

A: Lactose operon is an example of inducible operon in which the expression of downstream genes will…

Q: Which option is true when considering the regulation of cAMP and the Lac operon. If bacteria are in…

A: Introduction: An operon is a coordinated unit of genetic expression in a bacteria. Jacob and Monod…

Q: The following table lists 4 bacterial strains that are partial diploids for lac operon genes. Given…

A: Strain A: The concern strain in absence of lactose is not expressing the beta galactoosidase. It's…

Q: A full-length eukaryotic gene is inserted into a bacterial chromosome. The gene contains a complete…

A: The eukaryotic DNA is composed of coding and non-coding sequences, almost 90% are non-coding…

Q: Which of the following statements is not true of both the LacI and TrpR repressor proteins?a) an…

A: The objective of the question is to identify the statement that is not true for both LacI and TrpR…

Q: a. The original constitutive operator mutations in thelac operon were all base changes in o1. Why…

A: The original constitutive operator mutations made in the lac operon involved base changes in the O1.…

Q: Which of the following statements is true regarding the lys2-128d reporter?  Select all that apply.…

A: INTRODUCTION Lys2-128d is a reporter allele of the Spt- gene in Saccharomyces cerevisiae (yeast)…

Q: The trp operon in E. coli encodes enzymes essential for the biosynthesis of tryptophan. In the…

A: trp operon is a repressor operon found in E. coli bacteria, which consists of a group of genes that…

Q: Consider the lac operon of E. coli. Specifically, explain the following: a. Describe the overall…

A: The lac operon is a collection of genes located in the chromosome of Escherichia coli, and it…

Q: Which of the following lac operon genotypes would allow for functional versions of all the…

A: lac operon: - Inducible system - Both positive and negative regulation. - Involved in lactose…

Q: On its chromosome, an E. coli cell has a genotype of lacI− lacZ+ lacY+ lacA+. It has an F′ factor…

A: Lac operon is studied extensively by using mutant strains of E.coli. It is studied under the domain…

Q: Which protein(s) would be bound to the DNA of the lac operon in the presence of both lactose and…

A: INTRODUCTION Lac operon This is group of genes having a single promoter. It contain protein that…

Q: hich operons would be functional under these conditions?   What repressors would be made? (NOTE:…

A: Operon is a regulatory unit consisting of cluster of genes containing operator promoter and…

Q: e following genetic and cellular conditions.  You do not need to consider the role of CAP in your…

A: Upper operon -  Is is super repressor. Once it is present in the sequence, it can not be removed. If…

Q: A mutant strain of Salmonella bacteria carries a mutation of the rho protein that has fully activity…

A: In bacteria, two transcription termination mechanisms take place. Intrinsic termination, which is…

Q: cAMP binds to cAMP Receptor Protein (CRP), allowing CRP to bind to the promoter of the lac operon

A: Almost all bacteria such as E. coli contains lac operon which operates in utilization of lactose as…

Q: A lac operon containing one mutation was cloned into a plasmid, which was introduced by…

A: (a) The plasma i gene forms such a type of repressor that will not be able to bind the inducer.

Q: If a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or…

A: The lac operon or the lactose operon is a mechanism that is involved in the metabolism and transport…

Q: 1) The UR operon which is composed of sequences A, B, C, and D (which may or may not be genes of…

A: An operon may be considered as a group of certain genes which function together so as to regulate…

Q: High levels of transcription of the lactose operon require all of the following, EXCEPT: A) O the…

A: Lac operon is a gene regulatory mechanism associated with bacterial species while using lactose for…

Q: For the following types of transcriptional control, indicate whether the protein produced by the…

A: An inducible operon is one which is not transcribed. For transcription of the molecule, it requires…

Q: Imagine that a new strain of E. coli is discovered, and it contains the following novel genetic…

A: The lac operon is an operon, which is a collection of genes with a single promoter. The genes in the…

Q: You have an E.coli strain in which the lac operon is not expressed when glucose is absent and…

A: Introduction :- E. coli is a bacteria that lives in healthy people and animals' intestines. The…

Q: You have created a null mutation of tRNA synthase for trp-tRNA (the synthase enzyme attaches the trp…

A: A null mutation is a mutation that completely removes a gene or part of a gene.In this case, the…

Q: Examine the following theoretical operon (gen) in E. coli which contains structural genes (genC,…

A: Question 1:The genB gene's translation would be prematurely stopped by a nonsense mutation in the…

Q: Tryptophan (Trp) attenuation is an extra mechanism of regulation of trp operon when trp level is too…

A: Attenuation is a mechanism of control of the tryptophan (Trp) operon that occurs when the level of…

Q: ou have isolated different mutants (reg1 and reg2) causing constitutive expression of the emu operon…

A: The operon's regulation can take place positively or negatively. If the negative operator is bound…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.