You have an E.coli strain in which the lac operon is not expressed when glucose is absent and lactose is present. You have already conducted tests with partial diploids to rule out lacl, lacO, lacZ, lacY and lacA as the culprits, so you know that something must be wrong with the positive regulation system. Which of the following mutations could be causing this mutant phenotype? O cya- where glucose cannot bind adenylate cyclase O cap- where CAMP cannot bind to CAP M cap- where CAP cannot bind to the CAP binding site O Mutation in the CAP binding site that prevents CAP from binding O cya- where adenylate cyclase cannot turn ATP into CAMP
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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