Strain X-23 has a mutation in the lacI gene resulting in a lacI protein that cannot bind DNA. Otherwise all other parts of the operon are functional. Circle the least number of components for an F' plasmid that will restore normal regulation and function of the lacoperon in the resulting partial diploid. (may need more than one) (a) lacI+ (b) lacO+ (c) lacP+ (d) lacZ+ (e) None, cannot be restored
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Strain X-23 has a mutation in the lacI gene resulting in a lacI protein that cannot bind DNA. Otherwise all other parts of the operon are functional.
Circle the least number of components for an F' plasmid that will restore normal regulation and function of the lacoperon in the resulting partial diploid. (may need more than one)
(a) lacI+ (b) lacO+ (c) lacP+ (d) lacZ+ (e) None, cannot be restored
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