nion and burger lover, your friend would like to incorporate the cheems gene (“cheM”) from the rare Shiba Inu into sweet onions, in order to perfect the vegetable’s viability as a hamburger ingredient. To do so, she plans to clone the cheM gene (with an eukaryotic promoter) into the dOge plasmid. The dOge plasmid is capable of replicating in E. coli and also contains an
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
As an onion and burger lover, your friend would like to incorporate the cheems gene (“cheM”) from the rare Shiba Inu into sweet onions, in order to perfect the vegetable’s viability as a hamburger ingredient. To do so, she plans to clone the cheM gene (with an eukaryotic promoter) into the dOge plasmid. The dOge plasmid is capable of replicating in E. coli and also contains an ampicillin resistance gene, under a prokaryotic promoter, as a selective marker. Then, she plans to perform a trans-domain conjugation between E. coli (containing the plasmid with the cheM gene) and onion cells. However, you are not confident that this project will be successful, and point out some issues with the overall methodology. Identify two issues that will not allow the onion to express the cheM gene, based on the overall methodology described above.
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