A) Outline the experimental procedure for cloning a eukaryotic gene and expressing it in E. coli. Focus on the essential steps starting with eukaryotic gene amplification to transformation of E. coli cells B) Explain how insertional inactivation can help you identify the colonies that carry the plasmid with your eukaryotic gene of interest C) Plasmids containing antibiotic resistance genes are widely used in gene eloning and other molecular biology techniques. What would happen if the eukaryotic gene was inserted into an antibiotic resistance gene on the plasmid?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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A) Outline the experimental procedure for cloning a eukaryotic gene and expressing it in
E. coli. Focus on the essential steps starting with eukaryotic gene amplification to
transformation of E. coli cells
B) Explain how insertional inactivation can help you identify the colonies that carry
the plasmid with your eukaryotic gene of interest
C) Plasmids containing antibiotic resistance genes are widely used in gene cloning and
other molecular biology techniques. What would happen if the eukaryotic gene was inserted into
an antibiotic resistance gene on the plasmid?
Transcribed Image Text:A) Outline the experimental procedure for cloning a eukaryotic gene and expressing it in E. coli. Focus on the essential steps starting with eukaryotic gene amplification to transformation of E. coli cells B) Explain how insertional inactivation can help you identify the colonies that carry the plasmid with your eukaryotic gene of interest C) Plasmids containing antibiotic resistance genes are widely used in gene cloning and other molecular biology techniques. What would happen if the eukaryotic gene was inserted into an antibiotic resistance gene on the plasmid?
Expert Solution
Step 1

Genetic engineering has enabled us to engineer the genes according to the desired gene product. It is studied under the domain of biotechnology.

 

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