Transformation is best described as: Group of answer choices The integration of foreign DNA into a genome The uptake of naked DNA from the environment The transfer of DNA via a bacteriophage Transfer of a plasmid from one organism to another   B.)    What is the function of the araC gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on to daughter cells   C.) What is the function of the bla gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on to daughter cells

Q: We transformed E coli cells with a plasmid modified to contain a ‘virulence factor’ which would…

A: A virulance factor is a characteristic or molecule possessed by a microorganisms such as bacteria…

Q: Colonies/ug of plasmid) based on these results? O about 140O O about 800 O about 4000 O about 400…

A: INTRODUCTION Transformation efficiency is that the efficiency by which cells can take up…

Q: The diagram is given below shows conjugation between F + or Hfr donor cells with F-recipient cells.…

A: Since you have posted a question with multiple sub-parts, we will solve the first three subparts for…

Q: Write in detail about the effects of presence as well as absence of multiple cloning site and…

A: MULTIPLE CLONING SITE. Also known as polylinker region, is a DNA region found in plasmid which have…

Q: 2. Your lab mate, Beaker, tells you that they have run into some issues with expressing a gene on a…

A: Lac operon: Lac operon is the system of gene expression by which bacterial cells can synthesise and…

Q: 1.) Describe the components that are important to a cloning vector.

A: The term vector refers to the DNA molecules that act as transporting vehicle which carries foreign…

Q: In gene cloning, a vector is required to transform the gene of interest into host cell. State the…

A: In gene cloning, a vector is required to transform the gene of interest into the host cell. The…

Q: Provide a detailed description and hand drawn figure of each of the following. (1) Mismatch…

A: Although the genetic variation is important for evolution, the survival of the individual demands…

Q: For pGLO plasmid that contains araC gene and Ampr gene, what are the names of their promoters and…

A: pGLO plasmid  pGLO plasmid is a 5371bp genetically engineered plasmid to create genetically modified…

Q: Plasmid A carries an ampicillin resistant gene (bla) and a gene for production of a GFP protein;…

A: Plasmids are important tools for modifying and analyzing genes in genetic engineering and molecular…

Q: List the major steps that would be required to clone the human insulin gene into a plasmid and to…

A: Gene cloning is the isolation of DNA (deoxyribonucleic acid) sequence or gene of interest and…

Q: plate your cells on a master plate without any antibiotics.  After colonies appear, you pick and…

A: Recombinant DNA technology is helpful for different gene related experiments.

Q: For Figure 15-7, draw out a series of steps that could explain the origin of this large plasmid…

A: We need to provide the process leading to the origin of a large plasmid containing various…

Q: Consider the image below. This image shows plasmid DNA isolated through exactly the same method that…

A: Plasmid DNA In contrast to a cell's chromosomal DNA, plasmidsare tiny, circular, double-stranded DNA…

Q: A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A: Introduction :- Recombinant DNA technology ( also known as genetic engineering) involves the use of…

Q: Most bacteria which are used in molecular biology are ampicillin sensitive and recA. Plasmid usually…

A: Mostly ampicillin sensitive and recA- bacteria are used in molecular biology for the transformation.…

Q: What do you mean by vector plasmid?

A: Bacteria can be defined as the single-celled microscopic organism which is cosmopolitan as it is…

Q: A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for…

A: "Biotechnology" is the use of our knowledge of biological processes to the development of beneficial…

Q: A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A: A plasmid vector is a small, circular DNA molecule that is commonly used in molecular biology for…

Q: The properties that all plasmids share are that they1. all carry genes for antimicrobial…

A: Plasmids are extrachromosomal nucleic acids that are found generally outside the host's genetic…

Q: Discuss TWO (2) common methods you could utilize to transform your recombinant plasmids into the…

A: Recombinant plasmids can be transformed into bacterial cells in common molecular biology…

Q: A. Use this data to generate a circular map of the plasmid. Pst I: 10 kb Bam HI: 10 kb Sal I:…

A: Restriction endonucleases are enzymes that cut DNA at specific sites. Restriction endonucleases…

Q: plasmid has been modified such that it carries the gene for green fluorescent protein (GFP) under…

A: GFP protein is found in the photoorgans of Aequorea Victoria a jelly fish. It is a 28 kDa protein.…

Q: 1)What is plasmid?

A: The deoxyribonucleic acid (DNA) is the double-stranded molecule that is the genetic material in most…

Q: Where is the F plasmid located in an Hfr cell? Where is the F plasmid located in an F+ cell?

A: Prokaryotic cells are unicellular organisms that differ from multicellular organisms in composition…

Q: Using one of the options below, finish the following statement so that it is correct. Plasmids are…

A: Plasmids: A plasmid is a tiny, round, double-stranded DNA particle that is different from a cell's…

Q: You have a patient slated to undergo somatic cell gene therapy to alleviate a cystic fibrosis…

A: Answer: Cystic fibrosis is the result of homozygous recessive mutations in the CFTR gene.

Q: In a transformation experiment, a sample of competent bacteria was mixed with a plasmid containing…

A: In a bacterial colony some bacteria are resistant to an antibiotic and some are susceptible.…

Q: Evaluate the paragraph below and drag the labels to complete the sentences that discuss antibiotic…

A: Antibiotic resistance is a growing worldwide problem that threatens public health. As bacteria…

Q: Antibiotic resistance genes are often found on R plasmids (see Natural Gene Transfer and Antibiotic…

A: The substances that kill bacteria are known as antibiotics. As the antibiotics are used regularly…

Q: ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it…

A: First it has to be understood that : 1) the CAP-cAMP complex acts as a glucose sensor and is only…

Q: Explain how DNA configuration affects the migration of cut and uncut preparations of a plasmid?

A: There are three types of plasmid DNA conformations: supercoiled, open-circular (oc), and linear…

Q: 4) List 3 things that a potential vector must have in order to be useful. 5) Sometimes the gene for…

A: 1.Three things that a potential vector must have in order to be useful is viral replication origin,…

Q: A target gene for producing human insulin must be inserted into a plasmid before the transformation…

A: Option 2 is correct only 1 and 3 follows

Q: B. Next you isolate DNA from colonies and cut the DNA with the restriction enzymes you chose from…

A: A. As the plasmid contains ampicillin resistance gene, therefore, it confers resistance to amicillin…

Q: In Figure 5-15, how are each of the following genotypesproduced?a. F+ a− c. F− a+b. F− a− d. F+ a+

A: Recombination is a technique by which pieces of DNA are broken and recombined to produce new…

Q: How is an R plasmid similar to an F plasmid?

A:

Q: How does one perform a Southern blot and how does one performs a western blot. What can a Southern…

A: Southern blotting is the procedure that requires the transfer of separated fragments of DNA (DNA) to…

Q: create a concept map illustrating the relationships among these key terms: 1. Foreign DNA; 2.…

A: Recombinant DNA technology is the combining of DNA molecules from two different organisms. This…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.