Most bacteria which are used in molecular biology are ampicillin sensitive and recA. Plasmid usually carry an ampicillin resistance gene to allow for selection of bacteria which absorb them during transformation. Growth of a colony on antiobiotic medium indicates that the bacteria is carrying the plasmid with the gene for ampicillin resistance, but only if the bacteria is recA. If the bacteria were recA and took up a plasmid with the ampicillin gene, what could happen that would make ampicillin resistance useless as an indicator that the bacteria is carrying the plasmid?
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Most bacteria which are used in molecular biology are ampicillin sensitive and recA. Plasmid usually carry an ampicillin resistance gene to allow for selection of bacteria which absorb them during transformation. Growth of a colony on antiobiotic medium indicates that the bacteria is carrying the plasmid with the gene for ampicillin resistance, but only if the bacteria is recA. If the bacteria were recA and took up a plasmid with the ampicillin gene, what could happen that would make ampicillin resistance useless as an indicator that the bacteria is carrying the plasmid?
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