Homework Help is Here – Start Your Trial Now!
Science
Biology
After transformation you were asked to grow bacterial cells transformed with plasmid on a plate that had X-gal and ampicillin. X-Gal is often used as in indicator dye, which turns blue when metabolized by B-galactosidase protein and used to test if cloning experiments have worked. [Note look at the vector diagrams carefully] Briefly explain how you would find the bacterial cells that are transformed with the plasmid with the YFG inserted.

After transformation you were asked to grow bacterial cells transformed with plasmid on a plate that had X-gal and ampicillin. X-Gal is often used as in indicator dye, which turns blue when metabolized by B-galactosidase protein and used to test if cloning experiments have worked. [Note look at the vector diagrams carefully] Briefly explain how you would find the bacterial cells that are transformed with the plasmid with the YFG inserted.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question

After transformation you were asked to grow bacterial cells transformed with plasmid on a plate that had X-gal and ampicillin. X-Gal is often used as in indicator dye, which turns blue when metabolized by B-galactosidase protein and used to test if cloning experiments have worked. [Note look at the vector diagrams carefully]

Briefly explain how you would find the bacterial cells that are transformed with the plasmid with the YFG inserted.

**Plasmid Map: pUC18** This image represents a circular plasmid map for the pUC18 vector, which is 2686 base pairs (bps) in length. It is commonly used in cloning due to its features. Key Features: 1. **Amp (Ampicillin Resistance Gene)** - Denoted in green, providing ampicillin resistance. - Located on the left side of the plasmid. 2. **pMB1 ori (Origin of Replication)** - Shown in light blue. - Allows the plasmid to replicate within a bacterial cell. 3. **lacZ (LacZ Gene)** - Indicated in dark blue. - Contains a multiple cloning site (MCS) within, used for inserting foreign DNA. 4. **LacR Binding Site** - The regulatory site for the lac operon shown in red. - Includes the Plac (promoter). **Enzyme Sites:** The box on the right lists enzyme sites in a numerical order: 1. Site Number 6: Plac (Promoter) 2. Site Number 2 3. Site Number 3: Near the beginning of the LacZ gene 4. Site Number 5: Within the MCS 5. Site Number 4 6. Site Number 1 This vector is designed for high-copy replication, blue/white screening of recombinant clones, and easy plasmid manipulation due to its multiple cloning site.
Transcribed Image Text:**Plasmid Map: pUC18** This image represents a circular plasmid map for the pUC18 vector, which is 2686 base pairs (bps) in length. It is commonly used in cloning due to its features. Key Features: 1. **Amp (Ampicillin Resistance Gene)** - Denoted in green, providing ampicillin resistance. - Located on the left side of the plasmid. 2. **pMB1 ori (Origin of Replication)** - Shown in light blue. - Allows the plasmid to replicate within a bacterial cell. 3. **lacZ (LacZ Gene)** - Indicated in dark blue. - Contains a multiple cloning site (MCS) within, used for inserting foreign DNA. 4. **LacR Binding Site** - The regulatory site for the lac operon shown in red. - Includes the Plac (promoter). **Enzyme Sites:** The box on the right lists enzyme sites in a numerical order: 1. Site Number 6: Plac (Promoter) 2. Site Number 2 3. Site Number 3: Near the beginning of the LacZ gene 4. Site Number 5: Within the MCS 5. Site Number 4 6. Site Number 1 This vector is designed for high-copy replication, blue/white screening of recombinant clones, and easy plasmid manipulation due to its multiple cloning site.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS