E. coli strains diploid for the lac region were constructed by introducing a plasmid carrying the lac genes. The plasmid carries one copy of the lac region, and the chromosome carries the other copy. The two copies of the lac region have different genotypes, as shown in the chart below. Indicate whether the products of the lacy gene (permease) and the lacZ gene (ß-galactosidase) will be inducible, uninducible, or constitutive in each strain (assuming glucose is absent). lac region on plasmid lac region on chromosome permease B-galactosidase I-o+Z+Y- I+o+ Z-Y+ I+o+Z+Y- I+o°Z=Y+ I- oº Z+Y- I+o+ Z-Y+ Is o+ Z+Y- I+o+ Z-Y+ I+ oc Z+Y- Is o+ Z-Y+

Q: The map of the lac operon is shown below. Consider the following examples that include both haploids…

A: Lac operon is the segment of DNA which includes regulatory gene, structural gene, promoter gene and…

Q: In several experiments, you find these orders of genes getting transferred in from the chromosome…

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Q: For the Escherichia coli lactose operon genotypes listed, + indicates a functional component, -…

A: Permit me to elaborate on these lac operon strains' genotypes and phenotypes:  1. lacZ+ lacZ+ lacY+…

Q: Why is protein expression of recombinant DNA induced during the log phase of bacterial growth? Why…

A: Recombinant Protein is a protein that is encoded by a gene that has been cloned into a system that…

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Q: In the Avery, McLeod, McCarty Experiment where supernatant from heat killed, virulent S Strain…

A: Avery, McLeod and McCarty experiment was used to determine the genetic material and its nature on an…

Q: Parts A-C show the genotypes for partial diploid strains of the lac operon. Gene names have been…

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Q: Which gene encodes the enzyme at the upper boxed step in the pathway?   Which gene encodes the…

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Q: You have isolated a series of mutants affecting regulation of the lac operon. All of these are…

A: The lac (lactose) operon works in the prokaryotic cells and regulates the metabolism of the lactose.…

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Q: IPTG Absent IPTG Present B-Galactosidase Permease B- Permease Galactoßsidase + O+ z+ Y+ + IS ot z+…

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Q: Brenner’s m mutant phages (m1–m6) described inFig. 8.8 were suppressed when grown in suppressor(su−)…

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Q: A microbial geneticist isolates a new mutation in E. coliand wishes to map its chromosomal location.…

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Q: Suppose you have six strains of E. coli. One is wildtype, and each of the other five has a single…

A: Escherichia coli (E.coli) has the following five mutant strains. Lac Z-, lacy-, lacl-, Oc, and…

Q: Explain how the lac operon can be used to express a recombinant protein in an E. coli cell.

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Q: In E. coli, the gene bioD+ encodes an enzyme involved in biotin synthesis, and galK+ encodes an…

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Q: yeast cells, telomerase remains active and maintains telomeres of about 300 base pairs. Propose what…

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Q: The genotypes of five merozygote bacteria are shown below. In each set, the genotype of the lac…

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Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.