E. coli strains diploid for the lac region were constructed by introducing a plasmid carrying the lac genes. The plasmid carries one copy of the lac region, and the chromosome carries the other copy. The two copies of the lac region have different genotypes, as shown in the chart below. Indicate whether the products of the lacy gene (permease) and the lacZ gene (ß-galactosidase) will be inducible, uninducible, or constitutive in each strain (assuming glucose is absent). lac region on plasmid lac region on chromosome permease B-galactosidase I-o+Z+Y- I+o+ Z-Y+ I+o+Z+Y- I+o°Z=Y+ I- oº Z+Y- I+o+ Z-Y+ Is o+ Z+Y- I+o+ Z-Y+ I+ oc Z+Y- Is o+ Z-Y+

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**E. coli strains diploid for the lac region were constructed by introducing a plasmid carrying the lac genes. The plasmid carries one copy of the lac region, and the chromosome carries the other copy. The two copies of the lac region have different genotypes, as shown in the chart below. Indicate whether the products of the lacY gene (permease) and the lacZ gene (β-galactosidase) will be inducible, uninducible, or constitutive in each strain (assuming glucose is absent).**

| **lac region on plasmid** | **lac region on chromosome** | **permease** | **β-galactosidase** |
|-----------------------------|-------------------------------|--------------|-----------------------|
| I⁻ o⁺ Z⁺ Y⁻                    | I⁺ o⁺ Z⁻ Y⁺                      |              |                       |
| I⁺ o⁺ Z⁺ Y⁻                    | I⁺ oᶜ Z⁻ Y⁺                      |              |                       |
| I⁻ oᶜ Z⁺ Y⁻                    | I⁺ o⁺ Z⁻ Y⁺                      |              |                       |
| Iˢ o⁺ Z⁺ Y⁻                    | I⁺ o⁺ Z⁻ Y⁺                      |              |                       |
| I⁺ oᶜ Z⁺ Y⁻                    | Iˢ o⁺ Z⁻ Y⁺                      |              |                       |

*Explanation*:
- "I⁺" denotes the presence of the functional lacI repressor.
- "I⁻" denotes a non-functional lacI repressor.
- "Iˢ" denotes a super-repressor that cannot be inactivated by the inducer.
- "o⁺" denotes the functional lac operator.
- "oᶜ" denotes a constitutive operator that cannot bind to the repressor.
- "Z⁺" denotes the presence of functional β-galactosidase gene.
- "Z⁻" denotes the absence (non-functional) of β-galactosidase gene.
- "Y⁺" denotes the presence of functional permease gene.
- "Y⁻" denotes the absence (non-functional) of permease gene.
Transcribed Image Text:**E. coli strains diploid for the lac region were constructed by introducing a plasmid carrying the lac genes. The plasmid carries one copy of the lac region, and the chromosome carries the other copy. The two copies of the lac region have different genotypes, as shown in the chart below. Indicate whether the products of the lacY gene (permease) and the lacZ gene (β-galactosidase) will be inducible, uninducible, or constitutive in each strain (assuming glucose is absent).** | **lac region on plasmid** | **lac region on chromosome** | **permease** | **β-galactosidase** | |-----------------------------|-------------------------------|--------------|-----------------------| | I⁻ o⁺ Z⁺ Y⁻ | I⁺ o⁺ Z⁻ Y⁺ | | | | I⁺ o⁺ Z⁺ Y⁻ | I⁺ oᶜ Z⁻ Y⁺ | | | | I⁻ oᶜ Z⁺ Y⁻ | I⁺ o⁺ Z⁻ Y⁺ | | | | Iˢ o⁺ Z⁺ Y⁻ | I⁺ o⁺ Z⁻ Y⁺ | | | | I⁺ oᶜ Z⁺ Y⁻ | Iˢ o⁺ Z⁻ Y⁺ | | | *Explanation*: - "I⁺" denotes the presence of the functional lacI repressor. - "I⁻" denotes a non-functional lacI repressor. - "Iˢ" denotes a super-repressor that cannot be inactivated by the inducer. - "o⁺" denotes the functional lac operator. - "oᶜ" denotes a constitutive operator that cannot bind to the repressor. - "Z⁺" denotes the presence of functional β-galactosidase gene. - "Z⁻" denotes the absence (non-functional) of β-galactosidase gene. - "Y⁺" denotes the presence of functional permease gene. - "Y⁻" denotes the absence (non-functional) of permease gene.
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