O The lac operon in E.coli encodes enzymes necessary for the breakdown of lactose. For each enzyme (lac Z and lac Y), indicate with a + or-whether or not it is made when there is no lactose or when there is lactose. B-galactosidase (lac Z) No Lactose Permease (lac Y) No Lactose Lactose Genotype TP OcZ Y/I P O*ZY* I'POCZ Y*/I P* O©Z*Y• PP O'Z'Y/I P'O Z'Y* PP O Z Y*/IIP'OZ'Y* rp O°Z*Y/I P*O*ZY* IPO'Z'Y*/I* P*O©Z*Y• I'P'O*ZY*/I P*OʻZ*Y° Lactose

Q: cAMP level is directly affected by glucose that helps regulate the lac operon Okasaki Fragments are…

A: As per our guidelines we are instructed to answer only 3 subparts of a question. So for other…

Q: closely linked open reading frames (ORFs) have been identified on the E. coli chromosome and they…

A: E.coli being a prokaryote consists of an operon which is the cluster of genes that helps in the…

Q: In the trp operon, tryptophan itself serves as the effector for the trp repressor which causes it to…

A: trp operon is also known as Tryptophan operon An operon are gene clusters coding structural proteins…

Q: In fruit flies, the sex development gene contains six sections. If sections 1,2,3,5, and 6 are…

A: Fruit fly is commonly known as Drosophila melanogaster and is typically used in research owing to…

Q: Capacity to carry oxygen by sickle cell hemoglobin is reduced due to

A: Sickle cell disease is a group of disorders of blood generally inherited from  parents to their…

Q: Pick six “partial diploid” lac operon problems from the lac operon practice sheet.  (Those are the…

A: i) The repressor (l-d) is defective and can't binds with the operator. So, the system remains always…

Q: Pegard to bacteriophage lambda's decision to enter the lytic or lysogenic cycle, which of following…

A: INTRODUCTION A bacteriophage, or phage for brief , is a virus that infects bacteria. Like other…

Q: You are working with a laboratory strain of E.coli that contains a 10 base-pair deletion in the Lac…

A: Introduction of the concept: The given concept is based on the lac operon in E.coli. The operon…

Q: Adenylyl cyclase can generate CAMP for use as a messenger within eukaryotic cells to stimulate the…

A: According to the question, we have to check that the above statement is true or false. So, let us…

Q: the folid ing mutations would result in the highest level of lac operon transcription in the absence…

A: Lac operon is an operon required for the transport and metabolism of lactose in E. coli and many…

Q: The lac operon consists of three structural genes, lacZ, lacY and lacA that are transcribed as a…

A: Lac operon has *3 structural genes- lacZ, lacY, lacA *promoter, *operator, *terminator, *regulator.

Q: P₁0 ORF1 t₁ ORF2 ORF3 t₂ The diagram above illustrates an operon that is regulated by the RepR…

A: An operon is a group of genes and the regulatory elements that regulate the expression of those…

Q: 5' 3' ORF1 ORF2 ORF3 ORF4 ORF5 1. Above are pictured 2 operons, one that includes ORF1 and OFR2 and…

A: The production of RNA from DNA is known as transcription that occurs within the cytoplasm of…

Q: Lacl' mutant-Lactose is present I P lacl lacP laco lacz lacy lacA LEVEL 16-21 1 OF 4 Look at the lac…

A: The lac operon, is a group of genes that are involved in the metabolism of lactose in bacteria. The…

Q: IPTG can be used in the laboratory as a synthetic inducer of the lac operon, instead of lactose.…

A: Lactose operon is an example of inducible operon in which the expression of downstream genes will be…

Q: 12b. Fill in the following table about the Lac operon (LacZ gene) Environmental Condition + Glucose…

A: Gene regulation is the mechanism to control the amount of gene expression in the cells.

Q: nder what condition will there be more transcription initiation of the Trp operon? O Low tryptophan…

A: mAn operon is a cluster of genes that are transcribed together to give a single messenger RNA (mRNA)…

Q: 1 [Choose ] Choose Promoter Operator Regulatory Gene Active Repressor Protein Structural Genes 3…

A: Lac operon is basically a mechanism essential in the metabolism in the E.coli bacteria.

Q: that best fits the phrase below (1-20) Cis-acting B Post-transcriptional modification…

A: Francis Crick proposed central dogma. The RNA is formed from DNA with the help of enzyme RNA…

Q: Which of the following lac operon genotypes would allow for functional versions of all the…

A: lac operon: - Inducible system - Both positive and negative regulation. - Involved in lactose…

Q: The arabinose operon How does the araC protein regulate of the arabinose operon. How does its…

A: How arac protein regulate arabinose operon and how binding differ  If arabinose is absent, the dimer…

Q: Parts A-C in the table show the genotypes for two partial diploid strains of the lac operon. Gene…

A: A group of genes called the lac operon are involved in the bacterial metabolism of lactose. *Three…

Q: Describe the main functional differences between the TRP and the Lac operons. Main functional…

A: TRP stands for tryptophan. It is an essential amino acid that must be obtained from the diet and is…

Q: Select the correct statement about the trp operon: high levels of tryptophan promotes high…

A: Introduction :  Lac Operon is a collection of genes with a single promotor that encodes genes for…

Q: What are the operon fusion and the gene fusion? How can they be used for studying gene expression in…

A: Fusion gene is a hybrid gene formed from two previously independent genes or ORFs (Open Reading…

Q: An E. coli lac- mutant (a mutant that cannot grow in the medium containing lactose as only carbon…

A: Operon, is a bacterial and viral genetic regulation system in which genes coding for functionally…

Q: Lac Repressor A Operator Lac Y eene Promoter Lac Z eene Lac A gene В Lac Y eene Lac A gene Promoter…

A: Lac operon consists of a group of genes that codes for a protein that allows bacteria to use lactose…

Q: please match the following with the right answer here are the option

A: Solution that destabilize the cell wall/ membrane of the bacteria :- Ice bath Protein that gives the…

Q: 8. Why is it adaptive for a bacterium to not express the genes that encode for that lactose…

A: Bacteria are able to adapt to their environment by regulating the expression of genes that encode…

Q: 6. Indicate the appropriate SI unit and instrument to use to measure the following: a. Mass of a…

A: Introduction A living organism possesses vital energy to sustain life. The living organism can…

Q: Laz Z Lac Z Lạc Y Lac Y Genotype -lactose +lactose -lactose +lactose a. I+P+O+Z-Y+/F I+P+O^S Z+ Y-…

A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…

Q: explain this image for the ecoli bacteria mutation trpE

A: Introduction The trp operon is a type of operon system found in E. coli bacteria. The trp operon…

Q: You have replaced all of the tryptophan codons in the trp leader sequence with leucine codons. If…

A: A codon is a sequence of three nucleotides (DNA or RNA) that corresponds to a specific amino acid or…

Q: Explain how the lac operon can be used to express a recombinant protein in an E. coli cell.

A: Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: 2. The promoter (P) is the start site of transcription through the binding of RNA polymerase before…

A: The lac operon: The lac operon or the lactose operon is the most widely studied gene with respect to…

Q: The symbols A, B, C, D, E represent all the components of the lac operon, excluding LacA. The…

A: The lac operon regulates gene expression in lactose metabolism in E. coli. It is composed of a…

Q: Let’s suppose a DNA mutation changes the consensus sequence at the −35 site in a way that inhibits σ…

A: A sigma factor or specificity factor refers to the protein factor required for the transcription…

Q: According to the operon hypothesis, the Z and Y genes of E. coli are classified as structural genes,…

A: The Lac Operon is responsible for Lactose metabolism and transport in E.coli. This operon consists…

Q: For your 25ul ligation reaction: You need to add 160ng of your digested insert DNA (which is at a…

A: Given: Total volume - 25ul. The DNA need to be added is 160 ng. One ul contains 160 ng of digested…

Q: What are the effects of the following conditions on Lac operon of bacteria? Please do not forget to…

A: Lac operon is the inducible system of catabolic degradation of lactose. Lac operon is a combination…

Q: What type of mutation is shown below:

A: Structural chromosomal mutations refer to the changes in structure of a chromosome due to insertion,…

Q: A haploid E. coli cell has the genotype I ¯ P+ O+ Z+ Y ¯ where the I ¯ indicates that the repressor…

A: The question is asking us to determine which statements are correct about an E. coli cell with a…

Q: (iii) What is the function of the promoter in the bacterial operon?

A: The lactose operon (also abbreviated as the lac operon) is a group of genes present in E. coli and…

Q: Predict a possible phenotype from the following mutations in the Lac Operon: a deletion in…

A: An operon is a functioning unit of DNA that contains a cluster of genes under the control of a…

Q: Which diagram shows a system in which the lac operon genes are NOT going to be transcribed by the E.…

A: The prokaryotic gene expression is regulated by operon system that involves expression of polycystic…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.